PMID- 1511163
OWN - NLM
STAT- MEDLINE
DCOM- 19920929
LR  - 20071115
IS  - 0925-5710 (Print)
IS  - 0925-5710 (Linking)
VI  - 55
IP  - 2
DP  - 1992 Apr
TI  - Identification and characterization of specific receptors for the LD78 cytokine.
PG  - 131-7
AB  - LD78 is a small secreted protein that has a sequence similar to a number of other 
      polypeptides, including murine macrophage inflammatory protein 1 alpha (MIP-1 
      alpha), interleukin 8 (IL-8), Act-2, monocyte chemoattractant protein 1 (MCP-1), 
      and others. These polypeptides are members of a novel cytokine superfamily that 
      is involved in the inflammatory response, wound healing, hematopoiesis, and 
      tumorigenesis. Specific receptors for purified clonal LD78 protein were measured 
      using four cell lines (HL-60, U937, Jurkat, and MJ). 125I-labeled recombinant 
      LD78 bound most efficiently to U937 cells. We therefore characterized the 
      receptors as being on the surface of U937 cells. Binding reached an equilibrium 
      after incubation for 60 min at 4 degrees C. Scatchard analysis showed that there 
      were two classes of binding sites on U937 cells, high affinity sites (Kd = 5.3 x 
      10(-9) M) and low affinity sites (Kd = 9.3 x 10(-8) M), with the average number 
      of binding sites per cell being approximately 30,000 and approximately 90,000, 
      respectively. These receptors for LD78 were distinct from the receptors for 
      gamma-IFN and for IL-8. SDS-PAGE analysis of chemically crosslinked 125I-labeled 
      LD78 receptor complexes identified a single band of 52 kDa. The ability to detect 
      specific LD78 receptors should prove valuable in efforts to molecularly clone 
      these receptors and to dissect the biological actions of LD78.
FAU - Yamamura, Y
AU  - Yamamura Y
AD  - Second Department of Internal Medicine, Kumamoto University Medical School, 
      Japan.
FAU - Hattori, T
AU  - Hattori T
FAU - Ohmoto, Y
AU  - Ohmoto Y
FAU - Takatsuki, K
AU  - Takatsuki K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Int J Hematol
JT  - International journal of hematology
JID - 9111627
RN  - 0 (Chemokine CCL3)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Cross-Linking Reagents)
RN  - 0 (Cytokines)
RN  - 0 (Macrophage Inflammatory Proteins)
RN  - 0 (Monokines)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Recombinant Proteins)
RN  - 0 (macrophage inflammatory protein 1alpha receptor)
SB  - IM
MH  - Chemokine CCL3
MH  - Chemokine CCL4
MH  - Cross-Linking Reagents/pharmacology
MH  - Cytokines/*metabolism
MH  - Hematopoietic Stem Cells/metabolism
MH  - Humans
MH  - Macrophage Inflammatory Proteins
MH  - Monokines/*metabolism
MH  - Neoplasm Proteins/*metabolism
MH  - Neoplastic Stem Cells/metabolism
MH  - *Receptors, Chemokine
MH  - Receptors, Immunologic/drug effects/*metabolism
MH  - Recombinant Proteins/metabolism
MH  - Tumor Cells, Cultured/metabolism
EDAT- 1992/04/01 00:00
MHDA- 1992/04/01 00:01
CRDT- 1992/04/01 00:00
PHST- 1992/04/01 00:00 [pubmed]
PHST- 1992/04/01 00:01 [medline]
PHST- 1992/04/01 00:00 [entrez]
PST - ppublish
SO  - Int J Hematol. 1992 Apr;55(2):131-7.