PMID- 15111781
OWN - NLM
STAT- MEDLINE
DCOM- 20040615
LR  - 20190917
IS  - 1077-4114 (Print)
IS  - 1077-4114 (Linking)
VI  - 26
IP  - 5
DP  - 2004 May
TI  - A strategy to detect chromosomal abnormalities in children with acute 
      lymphoblastic leukemia.
PG  - 294-300
AB  - Conventional cytogenetics (CC) can be used to identify chromosomal abnormalities 
      that are predictors of treatment outcome in acute lymphoblastic leukemia (ALL). 
      The detection of abnormalities in ALL is difficult because low mitotic index and 
      poor-quality metaphases are obtained. Flow cytometry (FC) and fluorescence in 
      situ hybridization (FISH) can be used to detect aneuploidy in any phase of the 
      cell cycle, increasing the number of analyzable cells. The aim of this study was 
      to develop a strategy combining these methods to improve the frequency of 
      chromosome abnormality detection. One hundred children with newly diagnosed ALL 
      were included. CC and DNA content analysis by FC were performed in all patients. 
      The numerical abnormalities identified by both methods were compared and patients 
      were classified as concordant or discordant. FISH was used to support aneuploidy 
      results in discrepant cases using centromeric probes for the chromosomes most 
      frequently involved in aneuploidy. CC and FC showed high concordance (86%). 
      Fourteen cases were discrepant: nine showed hypodiploidy and low hyperdiploidy by 
      cytogenetics and five showed high hyperdiploidy by FC. FISH confirmed aneuploidy 
      in 12 cases in which it could be performed. High hyperdiploidy was the most 
      common abnormality; the 31 cases showing this aneuploidy were identified by FC. 
      The search for abnormalities must begin by measuring DNA content to detect this 
      aneuploidy, which is useful to evaluate the patient's risk. However, it is 
      important to screen for structural abnormalities by CC or molecular techniques. 
      This strategy may detect chromosomal abnormalities, optimizing resources in 
      laboratories where not all the screening methods are available.
FAU - Perez-Vera, Patricia
AU  - Perez-Vera P
AD  - Departamento de Investigacion en Genetica Humana, Instituto Nacional de 
      Pediatria, Mexico. ppverezvera@yahoo.com
FAU - Frias, Sara
AU  - Frias S
FAU - Carnevale, Alessandra
AU  - Carnevale A
FAU - Betancourt, Miguel
AU  - Betancourt M
FAU - Mujica, Marisa
AU  - Mujica M
FAU - Rivera-Luna, Roberto
AU  - Rivera-Luna R
FAU - Ortiz, Rocio
AU  - Ortiz R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Pediatr Hematol Oncol
JT  - Journal of pediatric hematology/oncology
JID - 9505928
SB  - IM
MH  - Adolescent
MH  - Age Factors
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - Cytogenetic Analysis
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Infant
MH  - Infant, Newborn
MH  - Karyotyping
MH  - Male
MH  - Methods
MH  - Molecular Diagnostic Techniques/*methods
MH  - Ploidies
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/classification/diagnosis/*genetics
MH  - Prognosis
EDAT- 2004/04/28 05:00
MHDA- 2004/06/16 05:00
CRDT- 2004/04/28 05:00
PHST- 2004/04/28 05:00 [pubmed]
PHST- 2004/06/16 05:00 [medline]
PHST- 2004/04/28 05:00 [entrez]
AID - 00043426-200405000-00007 [pii]
AID - 10.1097/00043426-200405000-00007 [doi]
PST - ppublish
SO  - J Pediatr Hematol Oncol. 2004 May;26(5):294-300. doi: 
      10.1097/00043426-200405000-00007.