PMID- 15113922
OWN - NLM
STAT- MEDLINE
DCOM- 20040527
LR  - 20190509
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 78
IP  - 10
DP  - 2004 May
TI  - Identification of a novel bovine herpesvirus 1 transcript containing a small open 
      reading frame that is expressed in trigeminal ganglia of latently infected 
      cattle.
PG  - 5438-47
AB  - Bovine herpesvirus 1 (BHV-1), like other Alphaherpesvirinae subfamily members, 
      establishes latency in sensory neurons. The latency-related (LR) RNA is 
      abundantly expressed during latency, and expression of an LR protein is required 
      for the latency reactivation cycle in cattle. Within LR promoter sequences, a 
      135-amino-acid open reading frame (ORF) was identified, ORF-E, that is antisense 
      to the LR RNA. ORF-E is also downstream of the gene encoding the major viral 
      transcriptional activator, bICP0. Strand-specific reverse transcription-PCR 
      demonstrated that a transcript containing ORF-E was consistently expressed in 
      trigeminal ganglia (TG) of latently infected calves, productively infected 
      cultured cells, and acutely infected calves. As expected, a late transcript 
      encoding glycoprotein C was not detected in TG of latently infected calves. The 
      ORF-E transcript is polyadenylated and is expressed early when cultured bovine 
      cells are productively infected. Protein coding sequences containing ORF-E were 
      fused to green fluorescent protein (GFP) to examine the cellular localization of 
      the putative protein. In transiently transfected mouse neuroblastoma (neuro-2A) 
      and human neuroblastoma (SK-N-SH) cells, the ORF-E/GFP fusion protein was 
      detected in discreet domains within the nucleus. In contrast, the ORF-E/GFP 
      fusion protein was detected in the cytoplasm and nucleus of rabbit skin cells and 
      bovine kidney cells. As expected, the GFP protein was expressed in the cytoplasm 
      and nucleus of transfected cells. These studies indicate that the ORF-E 
      transcript is consistently expressed during latency. We suggest that the ORF-E 
      gene regulates some aspect of the latency reactivation cycle.
FAU - Inman, Melissa
AU  - Inman M
AD  - Department of Veterinary and Biomedical Sciences, University of Nebraska, Lincoln 
      Fair Street at East Campus Loop, Lincoln, NE 68583-0905, USA.
FAU - Zhou, Joe
AU  - Zhou J
FAU - Webb, Heather
AU  - Webb H
FAU - Jones, Clinton
AU  - Jones C
LA  - eng
GR  - P20 RR015635/RR/NCRR NIH HHS/United States
GR  - 1P20 RR 15635/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Cattle
MH  - Cattle Diseases/*virology
MH  - Herpesviridae Infections/*veterinary/virology
MH  - Herpesvirus 1, Bovine/*genetics
MH  - Molecular Sequence Data
MH  - *Open Reading Frames
MH  - RNA, Messenger/*analysis
MH  - RNA, Viral/*analysis
MH  - Transfection
MH  - Trigeminal Ganglion/*virology
MH  - *Virus Latency
PMC - PMC400376
EDAT- 2004/04/29 05:00
MHDA- 2004/05/28 05:00
PMCR- 2004/05/01
CRDT- 2004/04/29 05:00
PHST- 2004/04/29 05:00 [pubmed]
PHST- 2004/05/28 05:00 [medline]
PHST- 2004/04/29 05:00 [entrez]
PHST- 2004/05/01 00:00 [pmc-release]
AID - 2456-03 [pii]
AID - 10.1128/jvi.78.10.5438-5447.2004 [doi]
PST - ppublish
SO  - J Virol. 2004 May;78(10):5438-47. doi: 10.1128/jvi.78.10.5438-5447.2004.