PMID- 15115145
OWN - NLM
STAT- MEDLINE
DCOM- 20040519
LR  - 20061115
IS  - 0125-1562 (Print)
IS  - 0125-1562 (Linking)
VI  - 34
IP  - 3
DP  - 2003 Sep
TI  - Comparative study of anti-double stranded DNA detection by ELISA and Crithidia 
      luciliae immunofluorescence.
PG  - 646-50
AB  - Two commercial enzyme-linked immunosorbent assays (ELISA) and two commercial 
      Crithidia luciliae immunofluorescence tests (CLIF) were reevaluated as to the 
      efficiency and degree of correlation of anti-double stranded DNA (anti-dsDNA) 
      detection in systemic lupus erythematosus (SLE). The two ELISAs exhibited an 
      overall agreement of 95% and significantly correlated with each other (r=0.91, 
      p<0.001). They were comparable in sensitivity (64%, 61%) and had the same 
      specificity (95%, 95%). The sensitivity of the two CLIFs was 39% and 29% with 
      corresponding specificities of 100% and 97%, and an overall agreement with each 
      other of 94%. The two ELISAs had comparable specificity to the CLIFs with good 
      agreement (84%, 79%) while they had a much greater sensitivity than the CLIFs. 
      These findings suggest that ELISA is a useful laboratory test for anti-dsDNA 
      detection of SLE due to its simplicity, quantitative results, sensitivity, 
      specificity and cost, as compared to CLIFs.
FAU - Janyapoon, Kritsana
AU  - Janyapoon K
AD  - Faculty of Medical Technology, Rangsit University, Pathum Thani, Thailand. 
      janya@hotmail.com
FAU - Jivakanont, Pantakit
AU  - Jivakanont P
FAU - Choosang, Kantima
AU  - Choosang K
FAU - Surbrsing, Rungkarn
AU  - Surbrsing R
FAU - Charoenying, Vannapa
AU  - Charoenying V
FAU - Baithong, Sirinya
AU  - Baithong S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Thailand
TA  - Southeast Asian J Trop Med Public Health
JT  - The Southeast Asian journal of tropical medicine and public health
JID - 0266303
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Reagent Kits, Diagnostic)
SB  - IM
MH  - Antibodies, Antinuclear/*analysis
MH  - Case-Control Studies
MH  - Enzyme-Linked Immunosorbent Assay/*methods
MH  - Fluorescent Antibody Technique/*methods
MH  - Humans
MH  - Linear Models
MH  - Lupus Erythematosus, Systemic/*diagnosis/genetics
MH  - Predictive Value of Tests
MH  - *Reagent Kits, Diagnostic
MH  - Sensitivity and Specificity
EDAT- 2004/04/30 05:00
MHDA- 2004/05/20 05:00
CRDT- 2004/04/30 05:00
PHST- 2004/04/30 05:00 [pubmed]
PHST- 2004/05/20 05:00 [medline]
PHST- 2004/04/30 05:00 [entrez]
PST - ppublish
SO  - Southeast Asian J Trop Med Public Health. 2003 Sep;34(3):646-50.