PMID- 15116295
OWN - NLM
STAT- MEDLINE
DCOM- 20040604
LR  - 20171116
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 189
IP  - 9
DP  - 2004 May 1
TI  - Dominance of CD86, transforming growth factor- beta 1, and interleukin-10 in 
      Mycobacterium tuberculosis secretory antigen-activated dendritic cells regulates 
      T helper 1 responses to mycobacterial antigens.
PG  - 1598-609
AB  - We report that stimulation of Mycobacterium tuberculosis (M. tuberculosis) 
      secretory antigen (MTSA)-differentiated dendritic cells (DCs) and MTSA-matured 
      DCs with M. tuberculosis cell extract (CE) down-regulated proinflammatory 
      responses to CE-primed T (CE-T) cells by increasing surface expression of CD86 
      after CE stimulation. CE stimulation also decreased interleukin (IL)-12p40 and 
      interferon (IFN)- gamma levels and increased IL-10 and transforming growth 
      factor- beta 1 (TGF- beta 1) levels from these DCs. Blocking either CD86, IL-10, 
      or TGF- beta with monoclonal antibodies before CE stimulation restored the 
      attenuated T helper 1 (Th1) responses of CE-T cells. Conversely, treatment of 
      these DCs with IL-12p70 and/or IFN- gamma completely restored Th1 responses from 
      CE-T cells. These results indicate that M. tuberculosis secretory antigens 
      down-regulate proinflammatory Th1 responses to mycobacteria by differentially 
      modulating the cytokine profiles and surface densities of costimulatory molecules 
      on DCs. Of importance, this down-regulation is independent of the maturation 
      status of MTSA-activated DCs and can be rescued after treatment of DCs with IFN- 
      gamma or IL-12.
FAU - Balkhi, Mumtaz Yaseen
AU  - Balkhi MY
AD  - Immunology Group, International Center for Genetic Engineering and Biotechnology, 
      Aruna Asaf Ali Marg, New Delhi, India.
FAU - Sinha, Aprajita
AU  - Sinha A
FAU - Natarajan, Krishnamurthy
AU  - Natarajan K
LA  - eng
GR  - AI-75320/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040416
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (Antigens, Bacterial)
RN  - 0 (Antigens, CD)
RN  - 0 (B7-2 Antigen)
RN  - 0 (Cd86 protein, mouse)
RN  - 0 (Cytokines)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Tgfb1 protein, mouse)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Antigens, Bacterial/immunology
MH  - Antigens, CD/*metabolism
MH  - B7-2 Antigen
MH  - Cell Differentiation
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology/metabolism
MH  - *Down-Regulation
MH  - Female
MH  - Interleukin-10/*metabolism
MH  - Membrane Glycoproteins/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mycobacterium tuberculosis/*immunology
MH  - Th1 Cells/*immunology
MH  - Transforming Growth Factor beta/*metabolism
MH  - Transforming Growth Factor beta1
EDAT- 2004/04/30 05:00
MHDA- 2004/06/05 05:00
CRDT- 2004/04/30 05:00
PHST- 2003/09/23 00:00 [received]
PHST- 2003/11/08 00:00 [accepted]
PHST- 2004/04/30 05:00 [pubmed]
PHST- 2004/06/05 05:00 [medline]
PHST- 2004/04/30 05:00 [entrez]
AID - JID31827 [pii]
AID - 10.1086/383328 [doi]
PST - ppublish
SO  - J Infect Dis. 2004 May 1;189(9):1598-609. doi: 10.1086/383328. Epub 2004 Apr 16.