PMID- 15117895
OWN - NLM
STAT- MEDLINE
DCOM- 20041221
LR  - 20071114
IS  - 0268-1161 (Print)
IS  - 0268-1161 (Linking)
VI  - 19
IP  - 6
DP  - 2004 Jun
TI  - Detection of structural and numerical chromosomal abnormalities by ACM-FISH 
      analysis in sperm of oligozoospermic infertility patients.
PG  - 1395-400
AB  - BACKGROUND: Modern reproductive technologies are enabling the treatment of 
      infertile men with severe disturbances of spermatogenesis. The possibility of 
      elevated frequencies of genetically and chromosomally defective sperm has become 
      an issue of concern with the increased usage of ICSI, which can enable men with 
      severely impaired sperm production to father children. Several papers have been 
      published reporting aneuploidy in oligozoospermic patients, but relatively little 
      is known about chromosome structural aberrations in the sperm of these patients. 
      METHODS: We examined sperm from infertile, oligozoospermic individuals for 
      structural and numerical chromosomal abnormalities using a multicolour ACM 
      fluorescence in situ hybridization (FISH) assay that utilizes DNA probes specific 
      for three regions of chromosome 1 to detect human sperm that carry numerical 
      chromosomal abnormalities plus two categories of structural aberrations: 
      duplications and deletions of 1pter and 1cen, and chromosomal breaks within the 
      1cen-1q12 region. RESULTS: There was a significant increase in the average 
      frequencies of sperm with duplications and deletions in the infertility patients 
      compared with the healthy concurrent controls. There was also a significantly 
      elevated level of breaks within the 1cen-1q12 region. There was no evidence for 
      an increase in chromosome 1 disomy, or in diploidy. CONCLUSIONS: Our data reveal 
      that oligozoospermia is associated with chromosomal structural abnormalities, 
      suggesting that oligozoospermic men carry a higher burden of transmissible, 
      chromosome damage. The findings raise the possibility of elevated levels of 
      transmissible chromosomal defects following ICSI treatment.
FAU - Schmid, T E
AU  - Schmid TE
AD  - Department of Biomedical Sciences, University of Bradford, UK.
FAU - Brinkworth, M H
AU  - Brinkworth MH
FAU - Hill, F
AU  - Hill F
FAU - Sloter, E
AU  - Sloter E
FAU - Kamischke, A
AU  - Kamischke A
FAU - Marchetti, F
AU  - Marchetti F
FAU - Nieschlag, E
AU  - Nieschlag E
FAU - Wyrobek, A J
AU  - Wyrobek AJ
LA  - eng
GR  - P4ZES04705/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040429
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
SB  - IM
MH  - Adult
MH  - *Chromosome Aberrations
MH  - Color
MH  - Gene Deletion
MH  - Genes, Duplicate
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence/methods
MH  - Infertility, Male/*genetics/pathology/physiopathology
MH  - Male
MH  - Oligospermia/*genetics/pathology/physiopathology
MH  - Sperm Count
MH  - Sperm Motility
MH  - Spermatozoa/*ultrastructure
EDAT- 2004/05/01 05:00
MHDA- 2004/12/22 09:00
CRDT- 2004/05/01 05:00
PHST- 2004/05/01 05:00 [pubmed]
PHST- 2004/12/22 09:00 [medline]
PHST- 2004/05/01 05:00 [entrez]
AID - deh278 [pii]
AID - 10.1093/humrep/deh278 [doi]
PST - ppublish
SO  - Hum Reprod. 2004 Jun;19(6):1395-400. doi: 10.1093/humrep/deh278. Epub 2004 Apr 
      29.