PMID- 15120186
OWN - NLM
STAT- MEDLINE
DCOM- 20041109
LR  - 20220310
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 65
IP  - 4
DP  - 2004 Apr
TI  - Regulation of FasL expression in natural killer cells.
PG  - 317-27
AB  - Fas ligand (FasL)-mediated cytotoxicity is initiated in natural killer (NK) cells 
      through ligation of their activating receptors. The CD16 receptor has been shown 
      to induce FasL expression and cytotoxicity in NK cells. In this study, we made 
      the novel observation that FasL expression was upregulated in NKL cells 
      stimulated through 2B4 and LFA-1 activating receptors, implying a role for 
      FasL-mediated cytotoxicity early in the immune response. Coligation with 
      CD94/NKG2A human leukocyte antigen (HLA) class I inhibitory receptor did not 
      block the induced FasL expression; therefore, these opposing pathways appear to 
      function independently. We also showed, however, that FasL-mediated cytotoxicity 
      was downregulated in CD94/NKG2A-expressing LAK cells in response to the HLA-E 
      ligand, suggesting a mechanism by which aberrant cells expressing class I may 
      evade FasL-mediated cytotoxicity. Thus we show for the first time that 2B4, 
      LFA-1, and CD94/NKG2A receptors are involved in modulating FasL expression and, 
      therefore, cytotoxicity mediated by NK cells.
FAU - Chua, Hui Lin
AU  - Chua HL
AD  - Department of Microbiology/Immunology, Indiana University School of Medicine, 
      Indianapolis, IN, USA.
FAU - Serov, Youri
AU  - Serov Y
FAU - Brahmi, Zacharie
AU  - Brahmi Z
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Antigens, CD)
RN  - 0 (CD244 protein, human)
RN  - 0 (FASLG protein, human)
RN  - 0 (Fas Ligand Protein)
RN  - 0 (HLA-A Antigens)
RN  - 0 (Interleukin-2)
RN  - 0 (Lymphocyte Function-Associated Antigen-1)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Signaling Lymphocytic Activation Molecule Family)
RN  - 0 (fas Receptor)
SB  - IM
MH  - Antigens, CD/immunology
MH  - Cytotoxicity, Immunologic/genetics/immunology
MH  - DNA Fingerprinting
MH  - DNA Fragmentation
MH  - Fas Ligand Protein
MH  - Flow Cytometry
MH  - HLA-A Antigens/immunology
MH  - Humans
MH  - Interleukin-2/immunology
MH  - Jurkat Cells
MH  - Killer Cells, Lymphokine-Activated/*immunology
MH  - Lymphocyte Function-Associated Antigen-1/immunology
MH  - Membrane Glycoproteins/*biosynthesis/genetics/immunology
MH  - RNA, Messenger/analysis
MH  - Receptors, Immunologic/immunology
MH  - Signaling Lymphocytic Activation Molecule Family
MH  - Up-Regulation
MH  - fas Receptor/immunology
EDAT- 2004/05/04 05:00
MHDA- 2004/11/13 09:00
CRDT- 2004/05/04 05:00
PHST- 2003/04/11 00:00 [received]
PHST- 2004/01/13 00:00 [revised]
PHST- 2004/01/14 00:00 [accepted]
PHST- 2004/05/04 05:00 [pubmed]
PHST- 2004/11/13 09:00 [medline]
PHST- 2004/05/04 05:00 [entrez]
AID - S0198885904000151 [pii]
AID - 10.1016/j.humimm.2004.01.004 [doi]
PST - ppublish
SO  - Hum Immunol. 2004 Apr;65(4):317-27. doi: 10.1016/j.humimm.2004.01.004.