PMID- 15120187
OWN - NLM
STAT- MEDLINE
DCOM- 20041109
LR  - 20101118
IS  - 0198-8859 (Print)
IS  - 0198-8859 (Linking)
VI  - 65
IP  - 4
DP  - 2004 Apr
TI  - Umbilical cord blood-naive T cells but not adult blood-naive T cells require HLA 
      class II on antigen-presenting cells for allo-immune activation.
PG  - 328-39
AB  - Because a relatively low incidence and severity of graft-versus-host disease 
      after umbilical cord blood (UCB) transplantation is observed, we investigated 
      whether T cells from UCB or adult blood (AB) were differentially activated by 
      antigen-presenting cells with or without human leukocyte antigen (HLA)-DR 
      expression. T cells from UCB or AB, or CD45RA(+) naive T cells and CD45RO(+) 
      memory T cells separated from AB, were stimulated with the HLA-DR(+) or HLA-DR(-) 
      cell line AML193. On days 1-3 after stimulation, numbers of interleukin (IL)-2, 
      IL-4, IL-10 or interferon gamma (IFN-gamma)-secreting cells were determined by 
      enzyme-linked immunospot analysis. No IL-4 or IL-10 was produced. AML193-DR(+) 
      cells induced IL-2 and IFN-gamma secretion with slower kinetics and lower levels 
      in UCB T cells than in AB T cells. AML193-DR(+) cells induced comparable IL-2 but 
      higher IFN-gamma secretion in CD45RA(+) T cells from AB than in UCB T cells. 
      AML193-DR(-) cells did not induce IL-2- or IFN-gamma secretion in UCB T cells, 
      but stimulated both CD45RA(+) and CD45RO(+) T cells from AB to secrete IL-2 and 
      IFN-gamma. Thus, not only the absence of memory T cells but also the inability to 
      respond to HLA-DR-negative antigen-presenting cells and the slower kinetics and 
      level of activation found for naive T cells from UCB as compared with AB may 
      partly explain the reduced antirecipient reactivity after UCB transplantation.
FAU - Kloosterboer, F M
AU  - Kloosterboer FM
AD  - Department of Hematology, Leiden University Medical Center, PO Box 9600, 2300 RC 
      Leiden, The Netherlands. F.M.Kloosterboer@lumc.nl
FAU - van Luxemburg-Heijs, S A P
AU  - van Luxemburg-Heijs SA
FAU - Willemze, R
AU  - Willemze R
FAU - Falkenburg, J H F
AU  - Falkenburg JH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (CD69 antigen)
RN  - 0 (Cytokines)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-D Antigens)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Interleukin-2)
RN  - 0 (Isoantigens)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Receptors, Interleukin-2)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Antigen-Presenting Cells/*immunology
MH  - Antigens, CD/immunology
MH  - Antigens, Differentiation, T-Lymphocyte/immunology
MH  - Cell Line
MH  - Cytokines/*biosynthesis
MH  - Fetal Blood/cytology/*immunology
MH  - HLA Antigens/*metabolism
MH  - HLA-A Antigens/immunology
MH  - HLA-D Antigens/immunology
MH  - Histocompatibility Antigens Class II/*metabolism
MH  - Humans
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-2/biosynthesis
MH  - Isoantigens/immunology
MH  - Lectins, C-Type
MH  - Receptors, Interleukin-2/immunology
MH  - T-Lymphocytes/*immunology
EDAT- 2004/05/04 05:00
MHDA- 2004/11/13 09:00
CRDT- 2004/05/04 05:00
PHST- 2003/10/06 00:00 [received]
PHST- 2003/12/30 00:00 [revised]
PHST- 2004/01/05 00:00 [accepted]
PHST- 2004/05/04 05:00 [pubmed]
PHST- 2004/11/13 09:00 [medline]
PHST- 2004/05/04 05:00 [entrez]
AID - S019888590400014X [pii]
AID - 10.1016/j.humimm.2004.01.003 [doi]
PST - ppublish
SO  - Hum Immunol. 2004 Apr;65(4):328-39. doi: 10.1016/j.humimm.2004.01.003.