PMID- 15121236
OWN - NLM
STAT- MEDLINE
DCOM- 20040706
LR  - 20131121
IS  - 0361-9230 (Print)
IS  - 0361-9230 (Linking)
VI  - 63
IP  - 1
DP  - 2004 Mar 1
TI  - Altered localization of choline transporter sites in the mouse hippocampus after 
      prenatal heroin exposure.
PG  - 25-32
AB  - Prenatal heroin exposure disrupts hippocampal cholinergic synaptic function and 
      related behaviors. Biochemical studies indicate an increase in the number of 
      presynaptic high-affinity choline transporter (HACT) sites, as assessed by 
      [3H]hemicholinium-3 (HC-3) binding. The present study was designed to assess 
      whether this effect involves global upregulation of the transporter, or whether 
      disruption occurs with a specific tempero-spatial distribution. Pregnant mice 
      were given 10mg/kg per day of heroin subcutaneously on gestational days (GD) 
      9-18. Autoradiographic distribution of HC-3 binding sites was evaluated in the 
      hippocampus of the offspring at postnatal days 15, 25, and 53. These results, 
      suggestive of hippocampal "miswiring," are likely to explain the net impairment 
      of cholinergic synaptic function after prenatal heroin exposure, despite the 
      simultaneous upregulation of both presynaptic cholinergic activity and 
      postsynaptic receptors. Understanding the subregional selectivity of hippocampal 
      defects can lead to the development of strategies that may potentially enable 
      therapeutic interventions to offset or reverse the neurobehavioral defects.
FAU - Vatury, Ori
AU  - Vatury O
AD  - The Ross Laboratory for Studies in Neural Birth Defects, Department of Anatomy 
      and Cell Biology, The Hebrew University-Hadassah Medical School, Box 12272, 91120 
      Jerusalem, Israel.
FAU - Barg, Jacob
AU  - Barg J
FAU - Slotkin, Theodore A
AU  - Slotkin TA
FAU - Yanai, Joseph
AU  - Yanai J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Brain Res Bull
JT  - Brain research bulletin
JID - 7605818
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (choline transporter)
RN  - 312-45-8 (Hemicholinium 3)
RN  - 70D95007SX (Heroin)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine/metabolism
MH  - Animals
MH  - Binding, Competitive/drug effects/physiology
MH  - Female
MH  - Hemicholinium 3/metabolism/pharmacokinetics
MH  - Heroin/*pharmacology
MH  - Heroin Dependence/*metabolism/physiopathology
MH  - Hippocampus/*drug effects/growth & development/metabolism
MH  - Male
MH  - Membrane Transport Proteins/*drug effects/metabolism
MH  - Mice
MH  - Neural Pathways/drug effects/physiopathology
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Presynaptic Terminals/drug effects/metabolism
MH  - Radioligand Assay
MH  - Synaptic Membranes/drug effects/metabolism
MH  - Synaptic Transmission/*drug effects/physiology
EDAT- 2004/05/04 05:00
MHDA- 2004/07/09 05:00
CRDT- 2004/05/04 05:00
PHST- 2003/09/07 00:00 [received]
PHST- 2003/11/15 00:00 [revised]
PHST- 2003/11/30 00:00 [accepted]
PHST- 2004/05/04 05:00 [pubmed]
PHST- 2004/07/09 05:00 [medline]
PHST- 2004/05/04 05:00 [entrez]
AID - S036192300300323X [pii]
AID - 10.1016/j.brainresbull.2003.11.004 [doi]
PST - ppublish
SO  - Brain Res Bull. 2004 Mar 1;63(1):25-32. doi: 10.1016/j.brainresbull.2003.11.004.