PMID- 15121508
OWN - NLM
STAT- MEDLINE
DCOM- 20040610
LR  - 20211018
IS  - 0091-6765 (Print)
IS  - 0091-6765 (Linking)
VI  - 112
IP  - 6
DP  - 2004 May
TI  - Urinary 8-hydroxy-2'-deoxyguanosine as a biomarker of oxidative DNA damage in 
      workers exposed to fine particulates.
PG  - 666-71
AB  - Residual oil fly ash (ROFA) is a chemically complex mixture of compounds, 
      including metals that are potentially carcinogenic because of their ability to 
      cause oxidative injury. In this study, we investigated the association between 
      exposure to particulate matter with an aerodynamic mass median diameter <or= 2.5 
      micro m (PM2.5) and oxidative DNA damage and repair, as indicated by urinary 
      8-hydroxy-2'-deoxyguanosine (8-OHdG) concentrations, in a group of boilermakers 
      exposed to ROFA and metal fumes. Twenty workers (50% smokers) were monitored for 
      5 days during an overhaul of oil-fired boilers. The median occupational PM2.5 
      8-hr time-weighted average was 0.44 mg/m3 (25th-75th percentile, 0.29-0.76). The 
      mean +/- SE creatinine-adjusted 8-OHdG levels were 13.26 +/- 1.04 micro g/g in 
      urine samples collected pre-workshift and 15.22 +/- 0.99 micro g/g in the 
      post-workshift samples. The urinary 8-OHdG levels were significantly greater in 
      the post-workshift samples than in the pre-workshift samples (p = 0.02), after 
      adjusting for urinary cotinine levels, chronic bronchitis status, and age. Linear 
      mixed models indicated a significant exposure-response association between PM2.5 
      exposure and urinary 8-OHdG levels (p = 0.03). Each 1-mg/m3 incremental increase 
      in PM2.5 exposure was associated with an increase of 1.67 micro g/g (95% 
      confidence interval, 0.21-3.14) in 8-OHdG levels. PM2.5 vanadium, manganese, 
      nickel, and lead exposures also were positively associated with 8-OHdG levels (p 
      <or= 0.05). This study suggests that a relatively young and healthy cohort of 
      boilermakers may experience an increased risk of developing oxidative DNA injury 
      after exposure to high levels of metal-containing particulate matter.
FAU - Kim, Jee Young
AU  - Kim JY
AD  - Department of Environmental Health, Occupational Health Program, Harvard School 
      of Public Health, Boston, Massachusetts 02115, USA.
FAU - Mukherjee, Sutapa
AU  - Mukherjee S
FAU - Ngo, Long C
AU  - Ngo LC
FAU - Christiani, David C
AU  - Christiani DC
LA  - eng
GR  - ES09860/ES/NIEHS NIH HHS/United States
GR  - T32 ES07069/ES/NIEHS NIH HHS/United States
GR  - ES00002/ES/NIEHS NIH HHS/United States
GR  - CA94715/CA/NCI NIH HHS/United States
GR  - T42110421/PHS HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Environ Health Perspect
JT  - Environmental health perspectives
JID - 0330411
RN  - 0 (Biomarkers)
RN  - 0 (Coal Ash)
RN  - 0 (Metals)
RN  - 0 (Particulate Matter)
RN  - 7440-44-0 (Carbon)
RN  - 88847-89-6 (8-Hydroxy-2'-Deoxyguanosine)
RN  - G9481N71RO (Deoxyguanosine)
SB  - IM
MH  - 8-Hydroxy-2'-Deoxyguanosine
MH  - Adolescent
MH  - Adult
MH  - Air Pollution, Indoor/*adverse effects
MH  - Biomarkers/*analysis
MH  - Carbon/*poisoning
MH  - Coal Ash
MH  - Cohort Studies
MH  - *DNA Damage
MH  - DNA Repair
MH  - Deoxyguanosine/*analogs & derivatives/*urine
MH  - Humans
MH  - Male
MH  - Metals/poisoning
MH  - Middle Aged
MH  - *Occupational Exposure
MH  - *Oxidative Stress
MH  - Particle Size
MH  - Particulate Matter
MH  - Risk Factors
PMC - PMC1241959
EDAT- 2004/05/04 05:00
MHDA- 2004/06/21 10:00
PMCR- 2004/05/01
CRDT- 2004/05/04 05:00
PHST- 2004/05/04 05:00 [pubmed]
PHST- 2004/06/21 10:00 [medline]
PHST- 2004/05/04 05:00 [entrez]
PHST- 2004/05/01 00:00 [pmc-release]
AID - 10.1289/ehp.6827 [doi]
PST - ppublish
SO  - Environ Health Perspect. 2004 May;112(6):666-71. doi: 10.1289/ehp.6827.