PMID- 1512205
OWN - NLM
STAT- MEDLINE
DCOM- 19920929
LR  - 20240109
IS  - 0021-9193 (Print)
IS  - 1098-5530 (Electronic)
IS  - 0021-9193 (Linking)
VI  - 174
IP  - 17
DP  - 1992 Sep
TI  - Isolation and characterization of Pseudomonas aeruginosa mutants blocked in the 
      synthesis of pyoverdin.
PG  - 5727-31
AB  - We have isolated and characterized by chemical and enzymatic analyses three 
      distinct types of pyoverdin-defective (pvd) mutants of Pseudomonas aeruginosa 
      PAO1. The pvd-1 mutant is an L-N5-hydroxyornithine (L-N5-OH-Orn) auxotroph unable 
      to hydroxylate L-ornithine (L-Orn) in a cell-free system and requiring 
      L-N5-OH-Orn for pyoverdin production. The other two types of mutants appear to be 
      blocked in further steps of the biosynthetic pathway leading to pyoverdin, 
      namely, the acylation of L-N5-OH-Orn (pvd-2) and chromophore synthesis (pvd-3). 
      The different pvd mutations were all found to be located in the catA1 region at 
      47 min of the genetic map of P. aeruginosa PAO1.
FAU - Visca, P
AU  - Visca P
AD  - Istituto di Microbiologia, Universita di Roma La Sapienza, Italy.
FAU - Serino, L
AU  - Serino L
FAU - Orsi, N
AU  - Orsi N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Bacteriol
JT  - Journal of bacteriology
JID - 2985120R
RN  - 0 (Oligopeptides)
RN  - 0 (Pigments, Biological)
RN  - 8062-00-8 (pyoverdin)
RN  - E524N2IXA3 (Ornithine)
SB  - IM
GS  - pvd-1
GS  - pvd-2
GS  - pvd-3
MH  - Chromosome Mapping
MH  - Genetic Complementation Test
MH  - Genetic Linkage
MH  - Mutation
MH  - *Oligopeptides
MH  - Ornithine/metabolism
MH  - Pigments, Biological/*biosynthesis
MH  - Pseudomonas aeruginosa/genetics/*metabolism
PMC - PMC206521
EDAT- 1992/09/01 00:00
MHDA- 1992/09/01 00:01
PMCR- 1992/09/01
CRDT- 1992/09/01 00:00
PHST- 1992/09/01 00:00 [pubmed]
PHST- 1992/09/01 00:01 [medline]
PHST- 1992/09/01 00:00 [entrez]
PHST- 1992/09/01 00:00 [pmc-release]
AID - 10.1128/jb.174.17.5727-5731.1992 [doi]
PST - ppublish
SO  - J Bacteriol. 1992 Sep;174(17):5727-31. doi: 10.1128/jb.174.17.5727-5731.1992.