PMID- 15122056
OWN - NLM
STAT- MEDLINE
DCOM- 20040511
LR  - 20170118
IS  - 1660-2137 (Electronic)
IS  - 1660-2137 (Linking)
VI  - 96
IP  - 4
DP  - 2004
TI  - Oxidative stress influences CC-chemokine levels in hemodialyzed patients.
PG  - p105-12
AB  - BACKGROUND: Increased oxidative stress (SOX) has been reported in hemodialyzed 
      (HD) patients, but its influence on CC-chemokine levels remains unknown. METHODS: 
      The levels of 3 distinct SOX markers (Cu/Zn superoxide dismutase (Cu/Zn SOD), 
      total lipid peroxide, and autoantibodies against oxidized LDL (OxLDL-Ab)), as 
      well as those of 4 CC-chemokines (monocyte chemoattractant protein-1 (MCP-1), 
      macrophage inflammatory proteins (MIP-1alpha, MIP-1beta), regulated upon 
      activation, normal T cell expressed and secreted (RANTES), and tumor necrosis 
      factor-alpha (TNF-alpha)) were measured pre- and post-HD session in 15 HD 
      patients with cardiovascular disease (CVD) and 12 HD patients without CVD (pre- 
      and post-HD session) and 17 controls. RESULTS: Cu/Zn SOD levels were elevated in 
      HD patients (both before and after HD) compared to controls (p < 0.001). Total 
      lipid peroxide levels were similar in the controls in pre-dialysis samples, but 
      were increased after a HD session (p < 0.001). Pre-dialysis OxLDL-Ab levels were 
      increased only in the HD group with CVD compared to controls (p < 0.05). The 
      pre-dialysis plasma levels of TNF-alpha (p < 0.001), MCP-1 (p < 0.001) and 
      MIP-1beta (p < 0.01) were increased both in all HD patients and subgroups with 
      CVD when compared to the controls, and remained significantly elevated when 
      measured after HD. Dialysis increased MCP-1 (p < 0.05) and MIP-1beta (p < 0.001) 
      levels as compared to the controls as well as in patients without CVD. Plasma 
      RANTES was significantly lower before HD (p < 0.05) and after HD (p < 0.001) in 
      patients with CVD. Patients without CVD and all HD patients also had lower RANTES 
      before and after HD when compared to controls (all p < 0.001). A positive 
      correlation was observed between plasma pre-dialysis Cu/Zn SOD levels and the 
      other SOX markers (all p < 0.05), age (p < 0.05) and duration of hemodialysis (p 
      < 0.001). A positive relationship existed between plasma Cu/Zn SOD levels and 
      those of MCP-1, MIP-1beta and TNF-alpha (all p < 0.001). RANTES levels negatively 
      correlated with Cu/Zn SOD (p < 0.0001).TNF-alpha positively correlated with age 
      (p < 0.05), total lipid peroxide (p = 0.010), MCP-1 (p < 0.01) and MIP-1beta 
      levels (p < 0.01). An identifiable association exists between MCP-1 and MIP-1beta 
      levels (p < 0.01), whereas both MCP-1 and MIP-1beta were inversely correlated 
      with RANTES (both p < 0.05). CONCLUSION: Our data indicate for the first time a 
      probable functional relationship between oxidative stress and CC-chemokine levels 
      in hemodialyzed patients, particularly in those with cardiovascular disease. This 
      relationship may represent one of the mechanisms involved in the progression of 
      atherosclerosis in these patients.
CI  - Copyright 2004 S. Karger AG, Basel
FAU - Pawlak, Krystyna
AU  - Pawlak K
AD  - Department of Nephrology, Medical University, Bialystok, Poland. 
      krystynapawlak@poczta.onet.pl
FAU - Pawlak, Dariusz
AU  - Pawlak D
FAU - Mysliwiec, Michal
AU  - Mysliwiec M
LA  - eng
PT  - Clinical Trial
PT  - Controlled Clinical Trial
PT  - Journal Article
PL  - Switzerland
TA  - Nephron Physiol
JT  - Nephron. Physiology
JID - 101159772
RN  - 0 (Autoantibodies)
RN  - 0 (Biomarkers)
RN  - 0 (Chemokines, CC)
RN  - 0 (Lipid Peroxides)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (oxidized low density lipoprotein)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Arteriosclerosis/immunology
MH  - Autoantibodies/blood
MH  - Biomarkers/blood
MH  - Cardiovascular Diseases/etiology
MH  - Chemokines, CC/*blood
MH  - Female
MH  - Humans
MH  - Kidney Failure, Chronic/blood/complications/therapy
MH  - Lipid Peroxides/blood
MH  - Lipoproteins, LDL/immunology
MH  - Male
MH  - Middle Aged
MH  - *Oxidative Stress
MH  - *Renal Dialysis
MH  - Superoxide Dismutase/blood
EDAT- 2004/05/04 05:00
MHDA- 2004/05/12 05:00
CRDT- 2004/05/04 05:00
PHST- 2003/03/09 00:00 [received]
PHST- 2004/02/09 00:00 [accepted]
PHST- 2004/05/04 05:00 [pubmed]
PHST- 2004/05/12 05:00 [medline]
PHST- 2004/05/04 05:00 [entrez]
AID - 77381 [pii]
AID - 10.1159/000077381 [doi]
PST - ppublish
SO  - Nephron Physiol. 2004;96(4):p105-12. doi: 10.1159/000077381.