PMID- 1512223
OWN - NLM
STAT- MEDLINE
DCOM- 19920925
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 267
IP  - 24
DP  - 1992 Aug 25
TI  - Biological activity of a proteoglycan form of macrophage colony-stimulating 
      factor and its binding to type V collagen.
PG  - 16812-5
AB  - Two different types of macrophage colony-stimulating factors (M-CSF) were found, 
      one with an apparent molecular mass of 85 kDa and the other greater than 200 kDa. 
      The high molecular mass M-CSF was identified as a proteoglycan carrying 
      chondroitin sulfate glycosaminoglycan and was designated as the proteoglycan form 
      of M-CSF (PG-M-CSF). In this study, we compared the biological activity of the 
      85-kDa M-CSF and PG-M-CSF and examined the binding properties of these two M-CSF 
      to certain extracellular matrix proteins, i.e. types I-V collagen and 
      fibronectin, using a modified enzyme-linked immunosorbent assay. PG-M-CSF was 
      capable of supporting the formation of murine macrophage colonies, and 
      pretreatment of PG-M-CSF with chondroitinase AC, which degrades chondroitin 
      sulfate, did not alter its colony-stimulating activity. The specific activity of 
      PG-M-CSF was similar to that of the 85-kDa M-CSF. The 85-kDa M-CSF had no 
      apparent affinity for the extracellular matrix proteins examined, whereas 
      PG-M-CSF had an appreciable binding capacity to type V collagen, but did not bind 
      to types I, II, III, and IV collagen or to fibronectin. Pretreatment of PG-M-CSF 
      with chondroitinase AC completely abolished the binding of the species to type V 
      collagen. Addition of exogenous chondroitin sulfate inhibited the binding of 
      PG-M-CSF to type V collagen in a dose-dependent manner. These data indicated that 
      the interaction between PG-M-CSF and type V collagen was mediated by the 
      chondroitin sulfate chain of PG-M-CSF. PG-M-CSF bound to type V collagen could 
      stimulate the proliferation of bone marrow macrophages, indicating that the 
      matrix protein-bound PG-M-CSF retained its biological activity. This interaction 
      between PG-M-CSF and type V collagen implies that the role of PG-M-CSF may be 
      distinct from that of 85-kDa M-CSF.
FAU - Suzu, S
AU  - Suzu S
AD  - Biochemical Research Laboratory, Morinaga Milk Industry Co, Ltd., Kanagawa, 
      Japan.
FAU - Ohtsuki, T
AU  - Ohtsuki T
FAU - Makishima, M
AU  - Makishima M
FAU - Yanai, N
AU  - Yanai N
FAU - Kawashima, T
AU  - Kawashima T
FAU - Nagata, N
AU  - Nagata N
FAU - Motoyoshi, K
AU  - Motoyoshi K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Proteoglycans)
RN  - 0 (Recombinant Proteins)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells
MH  - CHO Cells
MH  - Cell Division/drug effects
MH  - Cells, Cultured
MH  - Collagen/*metabolism
MH  - Cricetinae
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Kinetics
MH  - Macrophage Colony-Stimulating Factor/*metabolism/*pharmacology
MH  - Macrophages/*cytology/drug effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Proteoglycans/*metabolism/pharmacology
MH  - Recombinant Proteins/pharmacology
MH  - Swine
EDAT- 1992/08/25 00:00
MHDA- 1992/08/25 00:01
CRDT- 1992/08/25 00:00
PHST- 1992/08/25 00:00 [pubmed]
PHST- 1992/08/25 00:01 [medline]
PHST- 1992/08/25 00:00 [entrez]
AID - S0021-9258(18)41855-8 [pii]
PST - ppublish
SO  - J Biol Chem. 1992 Aug 25;267(24):16812-5.