PMID- 15122326
OWN - NLM
STAT- MEDLINE
DCOM- 20040805
LR  - 20061115
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 23
IP  - 33
DP  - 2004 Jul 22
TI  - E2F3 amplification and overexpression is associated with invasive tumor growth 
      and rapid tumor cell proliferation in urinary bladder cancer.
PG  - 5616-23
AB  - E2F3 is located in the 6p22 bladder amplicon and encodes a transcription factor 
      important for cell cycle regulation and DNA replication. To further investigate 
      the role of E2F3 in bladder cancer, a tissue microarray containing samples from 
      2317 bladder tumors was used for gene copy number and expression analysis by 
      means of fluorescence in situ hybridization (FISH) and immunohistochemistry 
      (IHC). E2F3 amplification was strongly associated with invasive tumor phenotype 
      and high tumor grade (P < 0.0001 each). None of 272 pTaG1/G2 tumors, but 35 of 
      311 pT1-4 carcinomas (11.3%), had E2F3 amplification. A high E2F3 expression 
      level was associated with high grade, advanced stage, and E2F3 gene amplification 
      (P < 0.0001 each). To evaluate whether E2F3 expression correlates with tumor 
      proliferation, the Ki67 labeling index (LI) was analysed for each tumor. There 
      was a strong association between a high Ki67 LI and E2F3 expression (P < 0.0001), 
      which was independent of grade and stage. We conclude that E2F3 is frequently 
      amplified and overexpressed in invasively growing bladder cancer (stage pT1-4). 
      E2F3 expression appears to provide a growth advantage to tumor cells by 
      activating cell proliferation in a subset of bladder tumors.
CI  - Copyright 2004 Nature Publishing Group
FAU - Oeggerli, Martin
AU  - Oeggerli M
AD  - Institute of Pathology, Schoenbeinstrasse 40, CH-4031 Basel, Switzerland.
FAU - Tomovska, Sanja
AU  - Tomovska S
FAU - Schraml, Peter
AU  - Schraml P
FAU - Calvano-Forte, Daniele
AU  - Calvano-Forte D
FAU - Schafroth, Salome
AU  - Schafroth S
FAU - Simon, Ronald
AU  - Simon R
FAU - Gasser, Thomas
AU  - Gasser T
FAU - Mihatsch, Michael J
AU  - Mihatsch MJ
FAU - Sauter, Guido
AU  - Sauter G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (E2F3 Transcription Factor)
RN  - 0 (E2F3 protein, human)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Cell Division
MH  - E2F3 Transcription Factor
MH  - *Gene Amplification
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Ki-67 Antigen/metabolism
MH  - Prognosis
MH  - Protein Array Analysis
MH  - Transcription Factors/*genetics/metabolism
MH  - Urinary Bladder Neoplasms/*genetics
EDAT- 2004/05/04 05:00
MHDA- 2004/08/06 05:00
CRDT- 2004/05/04 05:00
PHST- 2004/05/04 05:00 [pubmed]
PHST- 2004/08/06 05:00 [medline]
PHST- 2004/05/04 05:00 [entrez]
AID - 1207749 [pii]
AID - 10.1038/sj.onc.1207749 [doi]
PST - ppublish
SO  - Oncogene. 2004 Jul 22;23(33):5616-23. doi: 10.1038/sj.onc.1207749.