PMID- 15122328
OWN - NLM
STAT- MEDLINE
DCOM- 20040721
LR  - 20091119
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 23
IP  - 30
DP  - 2004 Jul 1
TI  - Overexpression of sprouty 2 inhibits HGF/SF-mediated cell growth, invasion, 
      migration, and cytokinesis.
PG  - 5193-202
AB  - A strict regulation of hepatocyte growth factor/scatter factor (HGF/SF)-Met 
      signaling is essential for its appropriate function. Several negative regulators 
      of Met signaling have been identified. Here we report that human Spry2 is induced 
      by HGF/SF and negatively regulates HGF/SF-Met signaling. We show that 
      overexpression of Spry2 inhibits cell proliferation, anchorage-independent cell 
      growth, and migration in wound-healing and in vitro invasion assays. Measured in 
      an electric cell-substrate impedance sensing biosensor, cell movement is 
      restricted, because Spry2 dramatically facilitates cell attachment and spreading 
      by enhancing focal adhesions and increasing stress fibers. An analysis of cell 
      cycle distribution shows, unexpectedly, that Spry2-GFP cells are polyploid. Thus, 
      as with FGF and EGF receptors, Spry2-GFP tempers downstream Met signaling in 
      addition to its pronounced effect on cell adhesion, and it has properties 
      suitable to be considered a tumor-suppressor protein.
FAU - Lee, Chong-Chou
AU  - Lee CC
AD  - Van Andel Research Institute, 333 Bostwick Avenue, N.E., Grand Rapids, MI 49503, 
      USA.
FAU - Putnam, Andrew J
AU  - Putnam AJ
FAU - Miranti, Cindy K
AU  - Miranti CK
FAU - Gustafson, Margaret
AU  - Gustafson M
FAU - Wang, Ling-Mei
AU  - Wang LM
FAU - Vande Woude, George F
AU  - Vande Woude GF
FAU - Gao, Chong-Feng
AU  - Gao CF
LA  - eng
PT  - Journal Article
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Drug Combinations)
RN  - 0 (HGF protein, human)
RN  - 0 (Laminin)
RN  - 0 (Luminescent Proteins)
RN  - 0 (Mitogens)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Proteoglycans)
RN  - 0 (Spry2 protein, rat)
RN  - 119978-18-6 (matrigel)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9007-34-5 (Collagen)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Biosensing Techniques
MH  - Blotting, Western
MH  - Carcinoma, Renal Cell/metabolism/pathology
MH  - Cell Adhesion
MH  - *Cell Division
MH  - Cell Line, Tumor
MH  - *Cell Movement
MH  - Collagen/metabolism
MH  - Drug Combinations
MH  - Focal Adhesions
MH  - *Gene Expression Regulation, Neoplastic
MH  - Green Fluorescent Proteins
MH  - Hepatocyte Growth Factor/*metabolism/pharmacology
MH  - Humans
MH  - Laminin/metabolism
MH  - Leiomyosarcoma/metabolism/pathology
MH  - Luminescent Proteins/metabolism
MH  - Mitogens/*metabolism/pharmacology
MH  - Neoplasm Invasiveness
MH  - Nerve Tissue Proteins/*metabolism
MH  - Polyploidy
MH  - Proteoglycans/metabolism
MH  - Proto-Oncogene Proteins c-met/metabolism
MH  - Signal Transduction
MH  - Stress Fibers
EDAT- 2004/05/04 05:00
MHDA- 2004/07/22 05:00
CRDT- 2004/05/04 05:00
PHST- 2004/05/04 05:00 [pubmed]
PHST- 2004/07/22 05:00 [medline]
PHST- 2004/05/04 05:00 [entrez]
AID - 1207646 [pii]
AID - 10.1038/sj.onc.1207646 [doi]
PST - ppublish
SO  - Oncogene. 2004 Jul 1;23(30):5193-202. doi: 10.1038/sj.onc.1207646.