PMID- 1512272
OWN - NLM
STAT- MEDLINE
DCOM- 19920925
LR  - 20210210
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 267
IP  - 24
DP  - 1992 Aug 25
TI  - Transforming growth factor beta differentially regulates expression of integrin 
      subunits in guinea pig airway epithelial cells.
PG  - 17409-14
AB  - The integrin family is composed of a large number of heterodimers, each one 
      mediating distinct interactions with extracellular matrix and/or cell surface 
      ligands. The expression of integrins appears to be tightly regulated in vivo, but 
      the mechanisms by which cells control the formation and surface expression of 
      specific pairs of subunits have not been well characterized. Two integrin 
      subunits, the alpha subunit alpha v, and the beta subunit beta 1, could pose 
      special problems in regulation because of their capacity to associate with 
      multiple partners. In the present study, we have examined the effects of the 
      cytokine transforming growth factor beta 1 (TGF-beta 1) on the expression of 
      alpha v- and beta 1-containing integrins in primary cultures of guinea pig airway 
      epithelial cells, e.g. cells that we have previously found to express multiple 
      potential partners for both alpha v and beta 1. TGF-beta 1 increased the surface 
      expression of both alpha v- and beta 1-containing heterodimers after periods of 
      stimulation from 24 to 72 h. These increases in surface expression were 
      associated with significant increases in the concentrations of mRNA encoding each 
      of the partners of alpha v and beta 1, but with only minimal increases in mRNA 
      encoding alpha v and beta 1 themselves. Airway epithelial cells metabolically 
      labeled with [35S]methionine during stimulation with TGF-beta 1 demonstrated only 
      a minimal increase in the synthesis of new alpha v protein at a time when 
      synthesis of alpha v's beta subunit partners and surface expression of alpha 
      v-containing heterodimers were dramatically increased. These data suggest that, 
      at least in some cells, promiscuous integrin subunits (both alpha and beta) may 
      normally be synthesized in excess. Thus, the surface expression of specific 
      integrin heterodimers can be regulated primarily through regulation of the 
      synthesis of the specific partners of these subunits.
FAU - Sheppard, D
AU  - Sheppard D
AD  - Lung Biology Center, University of California, San Francisco 94143-0854.
FAU - Cohen, D S
AU  - Cohen DS
FAU - Wang, A
AU  - Wang A
FAU - Busk, M
AU  - Busk M
LA  - eng
GR  - HL 19155/HL/NHLBI NIH HHS/United States
GR  - HL 47412/HL/NHLBI NIH HHS/United States
GR  - HL-AI33259/HL/NHLBI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Integrins)
RN  - 0 (Macromolecular Substances)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - AE28F7PNPL (Methionine)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Bronchi/*physiology
MH  - Cell Line
MH  - Cells, Cultured
MH  - Epithelium/drug effects/physiology
MH  - Gene Expression Regulation/*drug effects
MH  - Guinea Pigs
MH  - Integrins/*biosynthesis/genetics/isolation & purification
MH  - Kinetics
MH  - Macromolecular Substances
MH  - Male
MH  - Methionine/metabolism
MH  - RNA, Messenger/genetics/*metabolism
MH  - Trachea/*physiology
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 1992/08/25 00:00
MHDA- 1992/08/25 00:01
CRDT- 1992/08/25 00:00
PHST- 1992/08/25 00:00 [pubmed]
PHST- 1992/08/25 00:01 [medline]
PHST- 1992/08/25 00:00 [entrez]
AID - S0021-9258(18)41941-2 [pii]
PST - ppublish
SO  - J Biol Chem. 1992 Aug 25;267(24):17409-14.