PMID- 15124121
OWN - NLM
STAT- MEDLINE
DCOM- 20040625
LR  - 20070413
IS  - 0767-0974 (Print)
IS  - 0767-0974 (Linking)
VI  - 20
IP  - 4
DP  - 2004 Apr
TI  - [From the conception of the PRINS to its coronation].
PG  - 465-73
AB  - As a non-isotopic molecular cytogenetic technique, the primed in situ (PRINS) 
      labelling reaction represents a major technological progress achieved in the past 
      decade. It has become a routine technique for the microscopic visualization of 
      specific DNA sequences in cells and nuclei and constitutes a good alternative to 
      the fluorescence in situ hybridization (FISH) procedure. Among the multiple 
      advantages that characterize the PRINS technique, specificity, rapidity, 
      reliability, reproducibility, and cost-effectiveness can be mentioned. PRINS can 
      be in addition associated with other techniques like FISH, indirect 
      immunofluorescence, and nick translation. The most recent developments show the 
      great potential of this technique. Now PRINS can be used to study single-copy 
      genes and, consequently, can be routinely used to investigate deletions 
      associated with microdeletion syndromes. Therefore, the PRINS technique has the 
      potential to become a widely used molecular cytogenetic tool in clinics and 
      research. This short review presents how the PRINS technique contributed to 
      further the understanding of biological phenomena and describes the different 
      possibilities and applications of the PRINS method in several biological and 
      clinical fields (pre-implantation testing, prenatal, constitutional and oncologic 
      genetic diagnosis).
FAU - Krabchi, Kada
AU  - Krabchi K
AD  - Departement de Pediatrie, Faculte de medecine, Universite de Sherbrooke, Quebec, 
      Canada.
FAU - Lavoie, Josee
AU  - Lavoie J
FAU - Coullin, Philippe
AU  - Coullin P
FAU - Bronsard, Marc
AU  - Bronsard M
FAU - Pellestor, Franck
AU  - Pellestor F
FAU - Yan, Ju
AU  - Yan J
FAU - Drouin, Regen
AU  - Drouin R
LA  - fre
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
TT  - De la conception du PRINS a son couronnement.
PL  - France
TA  - Med Sci (Paris)
JT  - Medecine sciences : M/S
JID - 8710980
RN  - 0 (DNA, Neoplasm)
RN  - 0 (DNA, Viral)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Base Sequence
MH  - Chromosomes/genetics/ultrastructure
MH  - DNA/analysis
MH  - DNA, Neoplasm/analysis
MH  - DNA, Viral/analysis
MH  - Fetal Diseases/diagnosis/genetics
MH  - Fluorescent Antibody Technique
MH  - Genetic Diseases, Inborn/diagnosis/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Interphase
MH  - Metaphase
MH  - Preimplantation Diagnosis/methods
MH  - Prenatal Diagnosis/methods
MH  - *Primed In Situ Labeling/methods/trends
MH  - Sensitivity and Specificity
RF  - 51
EDAT- 2004/05/05 05:00
MHDA- 2004/06/26 05:00
CRDT- 2004/05/05 05:00
PHST- 2004/05/05 05:00 [pubmed]
PHST- 2004/06/26 05:00 [medline]
PHST- 2004/05/05 05:00 [entrez]
AID - 008123ar [pii]
AID - 10.1051/medsci/2004204465 [doi]
PST - ppublish
SO  - Med Sci (Paris). 2004 Apr;20(4):465-73. doi: 10.1051/medsci/2004204465.