PMID- 15124902
OWN - NLM
STAT- MEDLINE
DCOM- 20050125
LR  - 20190818
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 259
IP  - 1-2
DP  - 2004 Apr
TI  - Energy metabolism during cutaneous wound healing in immunocompromised and aged 
      rats.
PG  - 9-14
AB  - Cutaneous cells primarily depend upon carbohydrate metabolism for their energy 
      requirement during healing process. But, it may be greatly hampered during 
      various pathological and altered physiological conditions. The present study was 
      therefore undertaken to investigate the intermediate steps of energy metabolism 
      by measuring enzyme activities in the granulation tissues of immunocompromised 
      and aged rats following excision-type of cutaneous injury. The activities of key 
      regulatory enzymes hexokinase (HK), phosphofructokinase (PFK), lactate 
      dehydrogenase (LDH), citrate synthase (CS) and glucose-6 phosphate dehydrogenase 
      (G6PD) have been monitored in the wound tissues of immunocompromised and aged 
      rats at different time intervals (2, 7, 14 and 21 days) of postwounding. The 
      activities of HK and CS were found significantly decreased both in 
      immunocompromised and aged rats as compared to control subjects. However G6PD 
      exhibited an elevated activity at early stage followed by a decreased activity at 
      later phase of healing both in immunocompromised and aged rats. The PFK and LDH 
      demonstrated an upward trend in immunocompromised rats but a decreasing trend in 
      aged rats. Thus, the results suggest that significant alterations in the 
      activities of energy metabolizing enzymes in the granulation tissues in both 
      immunocompromised as well as in aged rats may overall affect the energy 
      availability for cellular activity needed for repair process. Hence, this may 
      perhaps be one of the factor responsible for impaired healing in these subjects.
FAU - Gupta, Asheesh
AU  - Gupta A
AD  - Division of Pharmacology, Central Drug Research Institute, PO Box No. 173, 
      Lucknow, India.
FAU - Manhas, Namratta
AU  - Manhas N
FAU - Raghubir, Ram
AU  - Raghubir R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.3.3.1 (Citrate (si)-Synthase)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - EC 2.7.1.11 (Phosphofructokinase-1)
RN  - WI4X0X7BPJ (Hydrocortisone)
SB  - IM
MH  - Aging/pathology/*physiology
MH  - Animals
MH  - Carbohydrate Metabolism
MH  - Citrate (si)-Synthase/metabolism
MH  - Energy Metabolism/*physiology
MH  - Glucosephosphate Dehydrogenase/metabolism
MH  - Granulation Tissue/enzymology
MH  - Hexokinase/metabolism
MH  - Hydrocortisone/administration & dosage
MH  - Immunocompromised Host/*physiology
MH  - Injections, Intramuscular
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Male
MH  - Phosphofructokinase-1/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Skin/*enzymology/pathology
MH  - Wound Healing/*physiology
EDAT- 2004/05/06 05:00
MHDA- 2005/01/26 09:00
CRDT- 2004/05/06 05:00
PHST- 2004/05/06 05:00 [pubmed]
PHST- 2005/01/26 09:00 [medline]
PHST- 2004/05/06 05:00 [entrez]
AID - 10.1023/b:mcbi.0000021339.34784.81 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2004 Apr;259(1-2):9-14. doi: 
      10.1023/b:mcbi.0000021339.34784.81.