PMID- 15125767
OWN - NLM
STAT- MEDLINE
DCOM- 20040709
LR  - 20091119
IS  - 0960-7412 (Print)
IS  - 0960-7412 (Linking)
VI  - 38
IP  - 4
DP  - 2004 May
TI  - Differential stage-specific regulation of cyclin-dependent kinases during cambial 
      dormancy in hybrid aspen.
PG  - 603-15
AB  - The cambium of woody plants cycles between active and dormant states. Dormancy 
      can be subdivided into eco- and endodormant stages. Ecodormant trees resume 
      growth upon exposure to growth-promotive signals, while the establishment of 
      endodormant state results in loss of the ability to respond to these signals. In 
      this paper, we analysed the regulation of cyclin-dependent kinases (CDKs) to 
      understand the differential response of cell division machinery to 
      growth-promotive signals during the distinct stages of dormancy in hybrid aspen. 
      We show that 4 weeks of short-day (SD) treatment causes termination of the 
      cambial cell division and establishment of the ecodormant state. This coincides 
      with a steady decline in the histone H1 kinase activity of the PSTAIRE-type 
      poplar CDKA (PttCDKA) and the PPTTLRE-type PttCDKB kinase complexes. However, 
      neither the transcript nor the polypeptide levels of PttCDKA and PttCDKB are 
      reduced during ecodormancy. In contrast, 6 weeks of SD treatment establishes 
      endodormancy, which is marked by the reduction and disappearance of the PttCDKA 
      and PttCDKB protein levels and the PttCDKB transcript levels. The transition to 
      endodormancy is preceded by an elevated E2F (adenosine E2 promoter binding 
      factor) phosphorylation activity of the PttCDKA kinase that reduces the 
      DNA-binding activity of E2F in vitro. The transition to endodormancy is followed 
      by a reduction of retinoblastoma (Rb) phosphorylation activity of PttCDKA protein 
      complexes. Both phosphorylation events could contribute to block the G1 to S 
      phase transition upon the establishment of endodormancy. Our results indicate 
      that eco- and endodormant stages of cambial dormancy involve a stage-specific 
      regulation of the cell cycle effectors at multiple levels.
FAU - Espinosa-Ruiz, Ana
AU  - Espinosa-Ruiz A
AD  - Department of Forest Genetics and Plant Physiology, Umea Plant Science Centre, 
      Swedish University of Agricultural Sciences, 90183 Umea, Sweden.
FAU - Saxena, Sangeeta
AU  - Saxena S
FAU - Schmidt, Julien
AU  - Schmidt J
FAU - Mellerowicz, Ewa
AU  - Mellerowicz E
FAU - Miskolczi, Pal
AU  - Miskolczi P
FAU - Bako, Laszlo
AU  - Bako L
FAU - Bhalerao, Rishikesh P
AU  - Bhalerao RP
LA  - eng
SI  - GENBANK/AF194820
SI  - GENBANK/AY307372
SI  - GENBANK/AY307373
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Plant Proteins)
RN  - EC 2.7.11.22 (Cyclin-Dependent Kinases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Binding Sites
MH  - Cloning, Molecular
MH  - Cyclin-Dependent Kinases/*genetics/metabolism
MH  - Gene Expression Regulation, Enzymologic/genetics
MH  - Gene Expression Regulation, Plant/*genetics
MH  - Kinetics
MH  - Light
MH  - Molecular Sequence Data
MH  - Phosphorylation
MH  - Plant Proteins/genetics/metabolism
MH  - Populus/enzymology/genetics/*physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Seasons
MH  - Wood
EDAT- 2004/05/06 05:00
MHDA- 2004/07/10 05:00
CRDT- 2004/05/06 05:00
PHST- 2004/05/06 05:00 [pubmed]
PHST- 2004/07/10 05:00 [medline]
PHST- 2004/05/06 05:00 [entrez]
AID - TPJ2070 [pii]
AID - 10.1111/j.1365-313X.2004.02070.x [doi]
PST - ppublish
SO  - Plant J. 2004 May;38(4):603-15. doi: 10.1111/j.1365-313X.2004.02070.x.