PMID- 15126053
OWN - NLM
STAT- MEDLINE
DCOM- 20041207
LR  - 20131121
IS  - 0143-4160 (Print)
IS  - 0143-4160 (Linking)
VI  - 36
IP  - 1
DP  - 2004 Jul
TI  - TRPV channels and modulation by hepatocyte growth factor/scatter factor in human 
      hepatoblastoma (HepG2) cells.
PG  - 19-28
AB  - Using patch clamp and Ca(2+) imaging techniques, we have studied Ca(2+) entry 
      pathways in human hepatoblastoma (HepG2) cells. These cells express the mRNA of 
      TRPV1, TRPV2, TRPV3 and TRPV4 channels, but not those of TRPV5 and TRPV6. 
      Functional assessment showed that capsaicin (10 microM), 
      4alpha-phorbol-12,13-didecanoate (4alphaPDD, 1 microM), arachidonic acid (10 
      microM), hypotonic stress, and heat all stimulated increases in [Ca(2+)](i) 
      within minutes. The increase in [Ca(2+)](i) depended on extracellular Ca(2+) and 
      on the transmembrane potential, which indicated that both driving forces affected 
      Ca(2+) entry. Capsaicin also stimulated an increase in [Ca(2+)](i) in nominally 
      Ca(2+)-free solutions, which was compatible with the receptor functioning as a 
      Ca(2+) release channel. Hepatocyte growth factor/scatter factor (HGF/SF) 
      modulated Ca(2+) entry. Ca(2+) influx was greater in HepG2 cells incubated with 
      HGF/SF (20 ng/ml for 20 h) compared with non-stimulated cells, but this occurred 
      only in those cells with a migrating phenotype as determined by presence of a 
      lamellipodium and trailing footplate. The effect of capsaicin on [Ca(2+)](i) was 
      greater in migrating HGF/SF-treated cells, and this was inhibited by capsazepine. 
      The difference between control and HGF/SF-treated cells was not found in 
      Ca(2+)-free solutions. 4alphaPDD also had no greater effect on HGF/SF-treated 
      cells. We conclude that TRPV1 and TRPV4 channels provide Ca(2+) entry pathways in 
      HepG2 cells. HGF/SF increases Ca(2+) entry via TRPV1, but not via TRPV4. This 
      rise in [Ca(2+)](i) may constitute an early response of a signalling cascade that 
      gives rise to cell locomotion and the migratory phenotype.
FAU - Vriens, Joris
AU  - Vriens J
AD  - Department of Physiology, Campus Gasthuisberg, Katholieke Universiteit, B-3000 
      Leuven, Belgium.
FAU - Janssens, Annelies
AU  - Janssens A
FAU - Prenen, Jean
AU  - Prenen J
FAU - Nilius, Bernd
AU  - Nilius B
FAU - Wondergem, Robert
AU  - Wondergem R
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Cell Calcium
JT  - Cell calcium
JID - 8006226
RN  - 0 (Cation Transport Proteins)
RN  - 0 (Hypotonic Solutions)
RN  - 0 (Ion Channels)
RN  - 0 (Phorbol Esters)
RN  - 0 (RNA, Messenger)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 54870-24-5 (4-O-methyl-phorbol-12,13-didecanoate)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - S07O44R1ZM (Capsaicin)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Arachidonic Acid/pharmacology
MH  - Calcium/metabolism/physiology
MH  - Capsaicin/pharmacology
MH  - Cation Transport Proteins/genetics/*physiology
MH  - Cell Line, Tumor
MH  - Cell Movement/*drug effects/physiology
MH  - Gene Expression
MH  - Hepatoblastoma
MH  - Hepatocyte Growth Factor/*pharmacology
MH  - Hepatocytes/drug effects/*metabolism
MH  - Hot Temperature
MH  - Humans
MH  - Hypotonic Solutions/pharmacology
MH  - Ion Channels/*classification/drug effects/*physiology
MH  - Models, Biological
MH  - Patch-Clamp Techniques
MH  - Phorbol Esters/pharmacology
MH  - RNA, Messenger/metabolism
MH  - Time Factors
EDAT- 2004/05/06 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/05/06 05:00
PHST- 2003/09/24 00:00 [received]
PHST- 2003/11/18 00:00 [revised]
PHST- 2003/11/19 00:00 [accepted]
PHST- 2004/05/06 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/05/06 05:00 [entrez]
AID - S0143416003002471 [pii]
AID - 10.1016/j.ceca.2003.11.006 [doi]
PST - ppublish
SO  - Cell Calcium. 2004 Jul;36(1):19-28. doi: 10.1016/j.ceca.2003.11.006.