PMID- 15126560
OWN - NLM
STAT- MEDLINE
DCOM- 20040714
LR  - 20191210
IS  - 0021-972X (Print)
IS  - 0021-972X (Linking)
VI  - 89
IP  - 5
DP  - 2004 May
TI  - Transfection of the multiple endocrine neoplasia type 1 gene to a human endocrine 
      pancreatic tumor cell line inhibits cell growth and affects expression of JunD, 
      delta-like protein 1/preadipocyte factor-1, proliferating cell nuclear antigen, 
      and QM/Jif-1.
PG  - 2326-37
AB  - In the absence of metastases or overgrowth to adjacent organs, the lack of 
      reliable markers for malignancy is a well-recognized problem for clinicians 
      managing patients with endocrine tumors. Apart from inactivation of the multiple 
      endocrine neoplasia type 1 (MEN1) gene, the molecular mechanisms involved in 
      tumorigenesis of the endocrine organs and MEN1-associated nonendocrine lesions 
      are vastly unknown. To try to learn more about down-stream effects on MEN1 gene 
      inactivation, we used the BON1 cells, showing low levels of endogenous menin, and 
      transfected them with a MEN1 gene construct. On restoring the menin expression, 
      we recorded inhibition of cell growth. We also performed macroarray and present 
      data on differentially expressed genes in the transfected cells, after 
      corroboration by Northern blots and quantitative PCR. JunD was up-regulated in 
      menin-expressing clones, whereas delta-like protein 1/preadipocyte factor-1 
      (involved in differentiation and growth of the pancreatic endocrine cells), 
      proliferating cell nuclear antigen, and QM/Jif-1 (a negative regulator of c-Jun) 
      became down-regulated. These findings might contribute to the understanding of 
      the tissue-specific features of MEN1. We also show that homozygous inactivation 
      of the MEN1 gene statistically correlates to higher expression of delta-like 
      protein 1/preadipocyte factor-1, proliferating cell nuclear antigen, and 
      QM/Jif-1, as well as lower MEN1 expression, in a limited sample of malignant 
      endocrine pancreatic tumors.
FAU - Stalberg, Peter
AU  - Stalberg P
AD  - Departments of Surgical Sciences, University Hospital, 751 85 Uppsala, Sweden.
FAU - Grimfjard, Per
AU  - Grimfjard P
FAU - Santesson, Marten
AU  - Santesson M
FAU - Zhou, Yinghua
AU  - Zhou Y
FAU - Lindberg, Daniel
AU  - Lindberg D
FAU - Gobl, Anders
AU  - Gobl A
FAU - Oberg, Kjell
AU  - Oberg K
FAU - Westin, Gunnar
AU  - Westin G
FAU - Rastad, Jonas
AU  - Rastad J
FAU - Wang, Shu
AU  - Wang S
FAU - Skogseid, Britt
AU  - Skogseid B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Clin Endocrinol Metab
JT  - The Journal of clinical endocrinology and metabolism
JID - 0375362
RN  - 0 (Avian Proteins)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Carrier Proteins)
RN  - 0 (DLK1 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Dlk1 protein, mouse)
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (MEN1 protein, human)
RN  - 0 (Membrane Proteins)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (RNA, Messenger)
RN  - 0 (RPL10 protein, human)
RN  - 0 (Repressor Proteins)
RN  - 0 (Ribosomal Proteins)
RN  - 0 (Rpl10 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 0 (jif-1 protein, chicken)
SB  - IM
MH  - Animals
MH  - Avian Proteins/genetics/*metabolism
MH  - Calcium-Binding Proteins
MH  - Carrier Proteins/genetics/*metabolism
MH  - Cell Division
MH  - Cell Line, Tumor
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins
MH  - Membrane Proteins/genetics/*metabolism
MH  - Mice
MH  - Mice, Nude
MH  - Neoplasm Transplantation
MH  - Pancreatic Neoplasms/*genetics/metabolism/pathology
MH  - Proliferating Cell Nuclear Antigen/genetics/*metabolism
MH  - Proto-Oncogene Proteins/*genetics
MH  - Proto-Oncogene Proteins c-jun/genetics/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Repressor Proteins/genetics/*metabolism
MH  - Ribosomal Protein L10
MH  - Ribosomal Proteins
MH  - Transcription Factors/genetics/metabolism
MH  - *Transfection
MH  - Transplantation, Heterologous
MH  - Tumor Suppressor Proteins/genetics/*metabolism
EDAT- 2004/05/06 05:00
MHDA- 2004/07/15 05:00
CRDT- 2004/05/06 05:00
PHST- 2004/05/06 05:00 [pubmed]
PHST- 2004/07/15 05:00 [medline]
PHST- 2004/05/06 05:00 [entrez]
AID - 10.1210/jc.2003-031228 [doi]
PST - ppublish
SO  - J Clin Endocrinol Metab. 2004 May;89(5):2326-37. doi: 10.1210/jc.2003-031228.