PMID- 15127159
OWN - NLM
STAT- MEDLINE
DCOM- 20040624
LR  - 20191210
IS  - 0179-7158 (Print)
IS  - 0179-7158 (Linking)
VI  - 180
IP  - 5
DP  - 2004 May
TI  - Impact of various parameters in detecting chromosomal aberrations by FISH to 
      describe radiosensitivity.
PG  - 289-96
AB  - BACKGROUND AND PURPOSE: Analysis of radiation-induced chromosomal aberrations is 
      regarded as the "gold standard" for classifying individual radiosensitivity. A 
      variety of different parameters can be used. The crucial question, however, is to 
      explore which parameter is suited best to describe the differences between 
      patients with increased radiosensitivity and healthy individuals. PATIENTS AND 
      METHODS: In this study, five patients with severe radiation-induced late effects 
      of at least grade 3, classified according to the Radiation Therapy Oncology Group 
      (RTOG), and eleven healthy individuals were examined retrospectively. Peripheral 
      blood lymphocytes were irradiated in vitro with 0.7 Gy and 2.0 Gy prior to 
      cultivation and stained by means of three-color fluorescence in situ 
      hybridization (FISH). The detailed analysis was focused on the number of breaks 
      per metaphase, on breaks from complex chromosomal rearrangements per metaphase, 
      as well as on the percentage of translocations, dicentric chromosomes, breaks, 
      and excess acentric fragments-each in comparison with the total number of mitoses 
      analyzed. RESULTS: Using the number of breaks from complex chromosomal 
      rearrangements after 2.0 Gy, radiosensitive patients as endpoint were clearly to 
      be distinguished (p = 0.001) from healthy individuals. Translocations (p = 0.001) 
      as well as breaks per metaphase (p = 0.002) were also suitable indicators for 
      detecting differences between patients and healthy individuals. The parameters 
      "percentage of dicentric chromosomes", "breaks", and "excess acentric fragments" 
      in comparison to the total number of mitoses analyzed could neither serve as 
      meaningful nor as significant criteria, since they showed a strong 
      interindividual variability. CONCLUSION: To detect a difference in chromosomal 
      aberrations between healthy and radiosensitive individuals, the parameters 
      "frequency of breaks per metaphase", "complex chromosomal rearrangements", and 
      "translocations" are most suitable.
FAU - Keller, Ulrike
AU  - Keller U
AD  - Division of Radiobiology, Department of Radiotherapy, Erlangen, Germany.
FAU - Kuechler, Alma
AU  - Kuechler A
FAU - Liehr, Thomas
AU  - Liehr T
FAU - Muller, Elisabeth
AU  - Muller E
FAU - Grabenbauer, Gerhard
AU  - Grabenbauer G
FAU - Sauer, Rolf
AU  - Sauer R
FAU - Distel, Luitpold
AU  - Distel L
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Validation Study
PL  - Germany
TA  - Strahlenther Onkol
JT  - Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et 
      al]
JID - 8603469
SB  - IM
MH  - Adult
MH  - Aged
MH  - Chromosome Aberrations/*radiation effects
MH  - Dose-Response Relationship, Radiation
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Leukocytes, Mononuclear/*radiation effects
MH  - Male
MH  - Middle Aged
MH  - Radiation Dosage
MH  - Radiation Injuries/*blood/classification/*genetics
MH  - Radiation Tolerance/*genetics/radiation effects
MH  - Reference Values
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
EDAT- 2004/05/06 05:00
MHDA- 2004/06/25 05:00
CRDT- 2004/05/06 05:00
PHST- 2003/06/04 00:00 [received]
PHST- 2003/12/19 00:00 [accepted]
PHST- 2004/05/06 05:00 [pubmed]
PHST- 2004/06/25 05:00 [medline]
PHST- 2004/05/06 05:00 [entrez]
AID - 10.1007/s00066-004-1200-y [doi]
PST - ppublish
SO  - Strahlenther Onkol. 2004 May;180(5):289-96. doi: 10.1007/s00066-004-1200-y.