PMID- 15127561
OWN - NLM
STAT- MEDLINE
DCOM- 20040727
LR  - 20161124
IS  - 0412-3948 (Print)
IS  - 0412-3948 (Linking)
VI  - 39
IP  - 1
DP  - 2004 Jan
TI  - [Detection of mutation in exon 5 and exon 8 of PTEN in laryngeal squamous cell 
      carcinoma].
PG  - 13-6
AB  - OBJECTIVE: To detect mutations of exon 5 and exon 8 of phosphatase and tensin 
      homology deleted on chromosome10/mutated in multiple advanced cancer1/TGF-beta 
      regulated and epithelial cell-enriched phosphatase (PTEN/MMAC1/TEP1 for short 
      PTEN) gene in laryngeal squamous cell carcinoma (LSCC) and analyze the 
      relationship between the mutation and LSCC. METHODS: Fresh tumor samples from 40 
      LSCC patients were examined using polymerase chain reaction-single-strand 
      conformation polymorphism (PCR-SSCP) and direct DNA sequencing. RESULTS: The 
      mutation of exon 5 and exon 8 occurred in supra-glottic laryngeal carcinoma and 
      no mutation in the glottic laryngeal carcinoma was found. There were 6 cases of 
      mutation for exon 5, and the mutation rate was 15%. In 2 cases, 1 base pair 
      insertion TT-->TAT in codon 85, while in other two cases, 1 base pair deletion 
      GCA-->GC in codon 86, and the two type mutation may result in frame shift 
      mutation. One case had the above two type mutation simultaneously, which may 
      result in missense mutation; 1 case had 1 base pair insertion TT-->TAT in 85 
      codon and 1 base pair deletion GCA-->GA, which may result in nonsense mutation. 
      The pathology of 3/6 (50%) cases was low differentiation, and the others (3/6, 
      50%) were middle differentiation. Five cases had lymphatic metastasis, and the 
      rate of which was 83.3%; the other one had no lymphatic metastasis, and the rate 
      of which was 16.7%. There was only 1 case mutation for exon 8, and the mutation 
      rate was 2.5%. One base pair deletion AAA-->AA in 276 codon as well as 1 base 
      pair insertion CT-->CCT between the 336 codon and 337 codon may result in frame 
      shift mutation. The pathology of the case was low differentiation. CONCLUSION: 
      The codon 85,86 of PTEN gene in exon 5 may be "hot spots" in LSCC and the 
      mutation of PTEN gene may be related with the lymphatic metastasis and middle or 
      low differentiation.
FAU - Bai, Wei-liang
AU  - Bai WL
AD  - Department of Otorhinolaryngology, Second Affiliated Hospital of China Medical 
      University, Shenyang 110004, China. baiweiliang@hotmail.com
FAU - Gao, Hong
AU  - Gao H
FAU - Ren, Zhong
AU  - Ren Z
FAU - Pan, Zi-min
AU  - Pan ZM
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Er Bi Yan Hou Ke Za Zhi
JT  - Zhonghua er bi yan hou ke za zhi
JID - 16210350R
RN  - 0 (DNA, Neoplasm)
RN  - EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
RN  - EC 3.1.3.48 (Protein Tyrosine Phosphatases)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Base Sequence
MH  - Carcinoma, Squamous Cell/*genetics
MH  - Chromosomes, Human, Pair 10
MH  - DNA Mutational Analysis
MH  - DNA, Neoplasm/analysis
MH  - Exons/genetics
MH  - Female
MH  - Genes, Tumor Suppressor
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Phosphoric Monoester Hydrolases/genetics
MH  - *Point Mutation
MH  - Polymorphism, Single-Stranded Conformational
MH  - Protein Tyrosine Phosphatases/*genetics
EDAT- 2004/05/07 05:00
MHDA- 2004/07/28 05:00
CRDT- 2004/05/07 05:00
PHST- 2004/05/07 05:00 [pubmed]
PHST- 2004/07/28 05:00 [medline]
PHST- 2004/05/07 05:00 [entrez]
PST - ppublish
SO  - Zhonghua Er Bi Yan Hou Ke Za Zhi. 2004 Jan;39(1):13-6.