PMID- 15128393
OWN - NLM
STAT- MEDLINE
DCOM- 20040614
LR  - 20220224
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 19
IP  - 9
DP  - 2004 May
TI  - Dental pulp cells provide neurotrophic support for dopaminergic neurons and 
      differentiate into neurons in vitro; implications for tissue engineering and 
      repair in the nervous system.
PG  - 2388-98
AB  - Glial cell line-derived neurotrophic factor (GDNF) mRNA is highly expressed by 
      dental pulp cells (DPCs) prior to the initiation of dental pulp innervation. We 
      show that radioactively labelled exogenous GDNF is retrogradely transported from 
      neonatal teeth and vibrissae to the trigeminal neurons, indicating that GDNF acts 
      as a classical neurotrophic factor in the trigeminal system. We also show that 
      DPCs from both rats and humans produce nerve growth factor (NGF), brain-derived 
      neurotrophic factor (BDNF) and GDNF mRNAs in vitro, promote the survival and 
      phenotypic characteristics of embryonic dopaminergic (DA) neurons and protect DA 
      neurons against the neurotoxin 6-hydroxy-dopamine (6-OHDA) in vitro. By using 
      inhibitory antibodies to NGF, BDNF and GDNF, we show that the promotion of DA 
      neuron survival relates to the production and release of neurotrophic proteins by 
      DPCs in vitro. We suggest that in vivo production of neurotrophic factors by DPCs 
      play roles in tooth innervation. However, continued production of neurotrophic 
      factors by the DPCs might have wider implications. We propose that the dental 
      pulp is a viable source of easily attainable cells with possible potential for 
      development of autologous cell transplantation therapies. We also show that a 
      population of neural crest-derived dental pulp cells acquire clear neuronal 
      morphology and protein expression profile in vitro, indicating the presence of a 
      cell population in the dental pulp with neuronal differentiation capacity that 
      might provide additional benefits when grafted into the CNS.
FAU - Nosrat, Irina V
AU  - Nosrat IV
AD  - Department of Biologic and Materials Sciences, Room 3218, School of Dentistry, 
      University of Michigan, Ann Arbor, MI 48109-1078, USA. nosrat@umich.edu
FAU - Smith, Christopher A
AU  - Smith CA
FAU - Mullally, Patrick
AU  - Mullally P
FAU - Olson, Lars
AU  - Olson L
FAU - Nosrat, Christopher A
AU  - Nosrat CA
LA  - eng
GR  - R03 DE014455-01A1/DE/NIDCR NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (Antibodies)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (DNA, Complementary)
RN  - 0 (GDNF protein, human)
RN  - 0 (Gdnf protein, rat)
RN  - 0 (Glial Cell Line-Derived Neurotrophic Factor)
RN  - 0 (Iodine Isotopes)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tubulin)
RN  - 0 (UCHL1 protein, human)
RN  - 8HW4YBZ748 (Oxidopamine)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Animals
MH  - Animals, Newborn
MH  - Antibodies/pharmacology
MH  - Brain-Derived Neurotrophic Factor/genetics/immunology/metabolism
MH  - Cell Count/methods
MH  - Cell Differentiation/*physiology
MH  - Cell Division/drug effects/physiology
MH  - Cell Survival/physiology
MH  - Cells, Cultured
MH  - Coculture Techniques/methods
MH  - DNA, Complementary/chemistry
MH  - Dental Pulp/cytology/innervation/*physiology
MH  - Dopamine/*metabolism
MH  - Embryo, Mammalian
MH  - Female
MH  - Glial Cell Line-Derived Neurotrophic Factor
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - Iodine Isotopes/pharmacokinetics
MH  - Male
MH  - Mesencephalon/cytology
MH  - Nerve Growth Factor/genetics/immunology/metabolism
MH  - Nerve Growth Factors/pharmacokinetics/*physiology
MH  - Neurons/drug effects/*metabolism
MH  - Oxidopamine/toxicity
MH  - Pregnancy
MH  - Pseudopodia/metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction/methods
MH  - Time Factors
MH  - Tissue Engineering
MH  - Tooth/embryology/innervation
MH  - Tubulin/metabolism
MH  - Tyrosine 3-Monooxygenase/metabolism
MH  - Ubiquitin Thiolesterase/metabolism
MH  - Vibrissae/embryology/innervation
EDAT- 2004/05/07 05:00
MHDA- 2004/06/15 05:00
CRDT- 2004/05/07 05:00
PHST- 2004/05/07 05:00 [pubmed]
PHST- 2004/06/15 05:00 [medline]
PHST- 2004/05/07 05:00 [entrez]
AID - EJN3314 [pii]
AID - 10.1111/j.0953-816X.2004.03314.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2004 May;19(9):2388-98. doi: 10.1111/j.0953-816X.2004.03314.x.