PMID- 15128404
OWN - NLM
STAT- MEDLINE
DCOM- 20040614
LR  - 20220309
IS  - 0953-816X (Print)
IS  - 0953-816X (Linking)
VI  - 19
IP  - 9
DP  - 2004 May
TI  - NQO1 activity in the main and the accessory olfactory systems correlates with the 
      zonal topography of projection maps.
PG  - 2511-8
AB  - The mouse olfactory epithelium (OE) is divided into spatial zones, each 
      containing neurons expressing zone-specific subsets of odorant receptor genes. 
      Likewise, the vomeronasal (VN) organ is organized into apical and basal 
      subpopulations of neurons expressing different VN receptor gene families. Axons 
      projecting from the different OE zones and VN subpopulations form synapses within 
      circumscribed regions in the glomerular layer of the olfactory bulb (OB) and 
      accessory olfactory bulb (AOB), respectively. We here show that mature neurons in 
      one defined zone selectively express NADPH:quinone oxidoreductase (NQO1), an 
      enzyme that catalyses reduction of quinones. Immunohistochemistry and in situ 
      hybridization analyses show non-overlapping expression of NQO1 and the Rb8 neural 
      cell adhesion molecule (RNCAM/OCAM) in OE and axon terminals within glomeruli of 
      the OB. In addition, NQO1 immunoreactivity reveals selective, zone-specific axon 
      fasciculation in the olfactory nerve. VN subpopulations do not show complementary 
      patterns of RNCAM and NQO1 immunoreactivity, instead both genes are co-expressed 
      in apical VN neurons that project to the rostral AOB. These results indicate that 
      one division of both the accessory and the main olfactory projection maps are 
      composed of sensory neurons that are specialized to reduce environmental and/or 
      endogenously produced quinones via an NQO1-dependent mechanism. The role of NQO1 
      in bioactivation of quinoidal drugs also points to a connection between 
      zone-specific NQO1 expression and zone-specific toxicity of certain olfactory 
      toxins.
FAU - Gussing, Fredrik
AU  - Gussing F
AD  - Department of Molecular Biology, Umea University, Umea, SE901 87, Sweden.
FAU - Bohm, Staffan
AU  - Bohm S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - France
TA  - Eur J Neurosci
JT  - The European journal of neuroscience
JID - 8918110
RN  - 0 (BACH2 protein, human)
RN  - 0 (Bach2 protein, mouse)
RN  - 0 (Basic-Leucine Zipper Transcription Factors)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (GAP-43 Protein)
RN  - 0 (GTP-Binding Protein alpha Subunits)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (Ncam2 protein, mouse)
RN  - 0 (Neural Cell Adhesion Molecules)
RN  - 0 (Nfe2l2 protein, mouse)
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factors)
RN  - 53-59-8 (NADP)
RN  - EC 1.6.5.2 (NAD(P)H Dehydrogenase (Quinone))
RN  - EC 1.6.5.2 (NQO1 protein, human)
SB  - IM
MH  - Animals
MH  - Basic-Leucine Zipper Transcription Factors
MH  - DNA-Binding Proteins/genetics/metabolism
MH  - GAP-43 Protein/metabolism
MH  - GTP-Binding Protein alpha Subunits/metabolism
MH  - Immunohistochemistry/methods
MH  - In Situ Hybridization/methods
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NAD(P)H Dehydrogenase (Quinone)/genetics/*metabolism
MH  - NADP/metabolism
MH  - NF-E2-Related Factor 2
MH  - Neural Cell Adhesion Molecules/metabolism
MH  - Neurons/*enzymology
MH  - Olfactory Bulb/cytology/*enzymology
MH  - Olfactory Mucosa/*cytology/enzymology
MH  - Trans-Activators/genetics/metabolism
MH  - Transcription Factors/genetics/metabolism
MH  - Vomeronasal Organ/cytology/enzymology
EDAT- 2004/05/07 05:00
MHDA- 2004/06/15 05:00
CRDT- 2004/05/07 05:00
PHST- 2004/05/07 05:00 [pubmed]
PHST- 2004/06/15 05:00 [medline]
PHST- 2004/05/07 05:00 [entrez]
AID - EJN3331 [pii]
AID - 10.1111/j.0953-816X.2004.03331.x [doi]
PST - ppublish
SO  - Eur J Neurosci. 2004 May;19(9):2511-8. doi: 10.1111/j.0953-816X.2004.03331.x.