PMID- 15128934
OWN - NLM
STAT- MEDLINE
DCOM- 20040706
LR  - 20220318
IS  - 0027-8424 (Print)
IS  - 1091-6490 (Electronic)
IS  - 0027-8424 (Linking)
VI  - 101
IP  - 20
DP  - 2004 May 18
TI  - HIV-1-infected monocyte-derived dendritic cells do not undergo maturation but can 
      elicit IL-10 production and T cell regulation.
PG  - 7669-74
AB  - Dendritic cells (DCs) undergo maturation during virus infection and thereby 
      become potent stimulators of cell-mediated immunity. HIV-1 replicates in immature 
      DCs, but we now find that infection is not accompanied by many components of 
      maturation in either infected cells or uninfected bystanders. The infected 
      cultures do not develop potent stimulating activity for the mixed leukocyte 
      reaction (MLR), and the DCs producing HIV-1 gag p24 do not express CD83 and 
      DC-lysosome-associated membrane protein maturation markers. If different 
      maturation stimuli are applied to DCs infected with HIV-1, the infected cells 
      selectively fail to mature. When DCs from HIV-1-infected patients are infected 
      and cultured with autologous T cells, IL-10 was produced in 6 of 10 patients. 
      These DC-T cell cocultures could suppress another immune response, the MLR. The 
      regulation was partially IL-10-dependent and correlated in extent with the level 
      of IL-10 produced. Suppressor cells only developed from infected patients, rather 
      than healthy controls, and the DCs had to be exposed to live virus rather than 
      HIV-1 gag peptides or protein. These results indicate that HIV-1-infected DCs 
      have two previously unrecognized means to evade immune responses: maturation can 
      be blocked reducing the efficacy of antigen presentation from infected cells, and 
      T cell-dependent suppression can be induced.
FAU - Granelli-Piperno, Angela
AU  - Granelli-Piperno A
AD  - Laboratory of Cellular Physiology and Immunology and Chris Browne Center for 
      Immunology and Immune Diseases, The Rockefeller University, 1230 York Avenue, New 
      York, NY 10021, USA. piperno@rockefeller.edu
FAU - Golebiowska, Angelika
AU  - Golebiowska A
FAU - Trumpfheller, Christine
AU  - Trumpfheller C
FAU - Siegal, Frederick P
AU  - Siegal FP
FAU - Steinman, Ralph M
AU  - Steinman RM
LA  - eng
GR  - R01 AI040045/AI/NIAID NIH HHS/United States
GR  - M01 RR000102/RR/NCRR NIH HHS/United States
GR  - P30 AI042848/AI/NIAID NIH HHS/United States
GR  - 5 P30 AI 42848-04/AI/NIAID NIH HHS/United States
GR  - R01 AI40045/AI/NIAID NIH HHS/United States
GR  - M0-1 RR00102/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040505
PL  - United States
TA  - Proc Natl Acad Sci U S A
JT  - Proceedings of the National Academy of Sciences of the United States of America
JID - 7505876
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Coculture Techniques
MH  - Dendritic Cells/physiology/*virology
MH  - HIV-1/*metabolism
MH  - HeLa Cells
MH  - Humans
MH  - Interleukin-10/*biosynthesis
MH  - T-Lymphocytes/*physiology
PMC - PMC419664
EDAT- 2004/05/07 05:00
MHDA- 2004/07/09 05:00
PMCR- 2004/11/18
CRDT- 2004/05/07 05:00
PHST- 2004/05/07 05:00 [pubmed]
PHST- 2004/07/09 05:00 [medline]
PHST- 2004/05/07 05:00 [entrez]
PHST- 2004/11/18 00:00 [pmc-release]
AID - 0402431101 [pii]
AID - 1017669 [pii]
AID - 10.1073/pnas.0402431101 [doi]
PST - ppublish
SO  - Proc Natl Acad Sci U S A. 2004 May 18;101(20):7669-74. doi: 
      10.1073/pnas.0402431101. Epub 2004 May 5.