PMID- 15131581
OWN - NLM
STAT- MEDLINE
DCOM- 20040617
LR  - 20171116
IS  - 0091-6749 (Print)
IS  - 0091-6749 (Linking)
VI  - 113
IP  - 5
DP  - 2004 May
TI  - Antigen-specific T cell-mediated apoptosis of dendritic cells is impaired in a 
      mouse model of food allergy.
PG  - 965-72
AB  - BACKGROUND: Dendritic cells (DCs) play a pivotal role in antigen presentation and 
      regulation of immune responses, and strong evidence suggests their involvement in 
      the pathogenesis of allergy. However, hitherto, DC-T-cell cross-talk in relation 
      to IgE-mediated allergic reactions to food has not been investigated. OBJECTIVE: 
      Our aim was to investigate T cell-mediated apoptosis of myeloid DCs from spleen 
      and Peyer's patches of mice with cow's milk (CM) allergy after cognate 
      interaction with antigen (CM)-specific T cells. METHODS: Freshly isolated myeloid 
      CD11c(+/hi)/B220(-) DCs from spleen and Peyer's patches of mice with CM allergy 
      and control mice were cultured with CM-specific T cells in the presence or 
      absence of CM or unrelated antigen as a control. Levels of apoptosis in DCs were 
      evaluated by assessing propidium iodide uptake and annexin V expression by means 
      of flow cytometry. RESULTS: We observed that both systemic and 
      gastrointestinal-derived DCs showed an increased resistance to T cell-mediated 
      cell death compared with DCs from control but not allergic donors. Further 
      experiments demonstrated that in both allergic and control mice, T cell-mediated 
      DC apoptosis takes place exclusively in the presence of the specific antigen, is 
      MHC II dependent, and is only partially CD95-CD95 ligand dependent. CONCLUSION: 
      Here we demonstrate, for the first time, that the reciprocal, finely balanced 
      regulation between these 2 cell types, which plays a central role in controlling 
      immune responses, is altered in allergy. We hypothesize that these events are 
      likely to have a profound influence on the genesis and maintenance of adverse 
      reaction to food.
FAU - Man, Angela L
AU  - Man AL
AD  - Laboratory of Gut Immunology, Programme of Gastrointestinal Health and Function, 
      Institute of Food Research, Colney, Norwich NR4 7UA, UK.
FAU - Bertelli, Eugenio
AU  - Bertelli E
FAU - Regoli, Mari
AU  - Regoli M
FAU - Chambers, Stephen J
AU  - Chambers SJ
FAU - Nicoletti, Claudio
AU  - Nicoletti C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Allergy Clin Immunol
JT  - The Journal of allergy and clinical immunology
JID - 1275002
RN  - 0 (Fas Ligand Protein)
RN  - 0 (Fasl protein, mouse)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (fas Receptor)
SB  - IM
CIN - J Allergy Clin Immunol. 2004 Jul;114(1):127-30. PMID: 15241355
MH  - Animals
MH  - Apoptosis/*immunology
MH  - Cattle
MH  - Dendritic Cells/*immunology/*pathology
MH  - Fas Ligand Protein
MH  - Female
MH  - Histocompatibility Antigens Class II/metabolism
MH  - In Vitro Techniques
MH  - Membrane Glycoproteins/metabolism
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Milk Hypersensitivity/*immunology/*pathology
MH  - Peyer's Patches/immunology/pathology
MH  - Spleen/immunology/pathology
MH  - T-Lymphocytes/*immunology
MH  - fas Receptor/metabolism
EDAT- 2004/05/08 05:00
MHDA- 2004/06/18 05:00
CRDT- 2004/05/08 05:00
PHST- 2004/05/08 05:00 [pubmed]
PHST- 2004/06/18 05:00 [medline]
PHST- 2004/05/08 05:00 [entrez]
AID - S009167490401070X [pii]
AID - 10.1016/j.jaci.2004.02.038 [doi]
PST - ppublish
SO  - J Allergy Clin Immunol. 2004 May;113(5):965-72. doi: 10.1016/j.jaci.2004.02.038.