PMID- 15132828
OWN - NLM
STAT- MEDLINE
DCOM- 20040810
LR  - 20161124
IS  - 1671-4083 (Print)
IS  - 1671-4083 (Linking)
VI  - 25
IP  - 5
DP  - 2004 May
TI  - Brain ischemia induces serine phosphorylation of neuronal nitric oxide synthase 
      by Ca(2+)/calmodulin-dependent protein kinase II in rat hippocampus.
PG  - 617-22
AB  - AIM: To investigate whether brain ischemia induces serine phosphorylation of 
      neuronal nitric oxide synthase (nNOS) by Ca(2+)/calmodulin-dependent protein 
      kinase II (CaMKII) and the interaction between CaMKII? and nNOS in rat 
      hippocampus. METHODS: Brain ischemia was induced by bilateral carotid artery 
      occlusion procedure. Phosphorylation and the interaction of proteins were studied 
      by immunoprecipitation and immunoblotting. We investigated during brain ischemia 
      serine phosphorylation and amount of nNOS in crude membranes fraction (P) and 
      cytosolic fraction (S), interaction between CaMKIIalpha and nNOS, and the effects 
      of 1-[N,O-bis-(5-isoquinolinesulfonyl)-N-methyl-L-tyrosyl]-4-phenylpiperazine 
      (KN-62, a selective inhibitor of CaMKII) on phosphorylation and the interaction 
      of proteins in P. RESULTS: Serine phosphorylation of nNOS in P increased 
      persistently during brain ischemia, and 15 min ischemia-induced serine 
      phosphorylation of nNOS was attenuated significantly by KN-62. But there was no 
      serine phosphorylation of nNOS in S. The distributions of nNOS were not affected 
      by ischemia and KN-62. However, the binding levels of both CaMKIIalpha with nNOS 
      and Thr(286) autophosphorylated CaMKIIalpha with nNOS increased after ischemia, 
      and were diminished by KN-62. CONCLUSION: CaMKII interacted with nNOS and 
      regulated serine phosphorylation of nNOS during brain ischemia.
FAU - Yan, Xue-bo
AU  - Yan XB
AD  - Research Center for Biochemistry and Molecular Biology, Xuzhou Medical College, 
      Xuzhou 221002, China.
FAU - Meng, Fan-jie
AU  - Meng FJ
FAU - Song, Bo
AU  - Song B
FAU - Zhang, Guang-yi
AU  - Zhang GY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Acta Pharmacol Sin
JT  - Acta pharmacologica Sinica
JID - 100956087
RN  - 452VLY9402 (Serine)
RN  - 63HM46XPOW (KN 62)
RN  - 84477-87-2 (1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type I)
RN  - EC 1.14.13.39 (Nos1 protein, rat)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinases)
SB  - IM
MH  - 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine/*analogs & derivatives/pharmacology
MH  - Animals
MH  - Brain Ischemia/*enzymology/metabolism
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2
MH  - Calcium-Calmodulin-Dependent Protein Kinases/antagonists & inhibitors/*metabolism
MH  - Hippocampus/*enzymology
MH  - Male
MH  - Nitric Oxide Synthase/*metabolism
MH  - Nitric Oxide Synthase Type I
MH  - Phosphorylation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Serine/*metabolism
EDAT- 2004/05/11 05:00
MHDA- 2004/08/11 05:00
CRDT- 2004/05/11 05:00
PHST- 2004/05/11 05:00 [pubmed]
PHST- 2004/08/11 05:00 [medline]
PHST- 2004/05/11 05:00 [entrez]
PST - ppublish
SO  - Acta Pharmacol Sin. 2004 May;25(5):617-22.