PMID- 15133080
OWN - NLM
STAT- MEDLINE
DCOM- 20040709
LR  - 20200826
IS  - 1350-0872 (Print)
IS  - 1350-0872 (Linking)
VI  - 150
IP  - Pt 5
DP  - 2004 May
TI  - Intron-containing beta-tubulin transcripts in Cryptosporidium parvum cultured in 
      vitro.
PG  - 1191-1195
LID - 10.1099/mic.0.26897-0 [doi]
AB  - The genome of Cryptosporidium parvum contains a relatively small number of 
      introns, which includes the beta-tubulin gene with only a single intron. 
      Recently, it was observed that the intron was not removed from some of the 
      beta-tubulin transcripts in the late life cycle stages cultured in vitro. 
      Although normally spliced beta-tubulin mRNA was detected in all parasite 
      intracellular stages by RT-PCR (e.g. HCT-8 or Caco-2 cells infected with C. 
      parvum for 12-72 h), at 48-72 h post-infection unprocessed beta-tubulin 
      transcripts containing intact introns started to appear in parasite mRNA within 
      infected host cells. The intron-containing transcripts could be detected by 
      fluorescence in situ hybridization (FISH) using an intron-specific probe. The 
      intron-containing beta-tubulin transcripts appeared unique to the in 
      vitro-cultured C. parvum, since they were not detected in parasite-infected 
      calves at 72 h. As yet, it is unclear whether the late life cycle stages of C. 
      parvum are partially deficient in intron-splicing or the intron-splicing 
      processes have merely slowed, both of which would allow the detection of 
      intron-containing transcripts. Another possible explanation is that the decay in 
      transcript processing might simply be due to the onset of parasite death. 
      Nonetheless, the appearance of intron-containing transcripts coincides with the 
      arrest of C. parvum development in vitro. This unusual observation prompts 
      speculation that the abnormal intron-splicing of beta-tubulin transcripts may be 
      one of the factors preventing complete development of this parasite in vitro. 
      Furthermore, the presence of both processed and unprocessed introns in 
      beta-tubulin transcripts in vitro may provide a venue for studying overall 
      mechanisms for intron-splicing in this parasite.
FAU - Cai, Xiaomin
AU  - Cai X
AD  - Department of Veterinary Pathobiology, College of Veterinary Pathobiology, Texas 
      A&M University, 4467 TAMU, College Station, TX 77843-4467, USA.
FAU - Lancto, Cheryl A
AU  - Lancto CA
AD  - Department of Veterinary Pathobiology, University of Minnesota, St Paul, MN 
      55108, USA.
FAU - Abrahamsen, Mitchell S
AU  - Abrahamsen MS
AD  - Department of Veterinary Pathobiology, University of Minnesota, St Paul, MN 
      55108, USA.
FAU - Zhu, Guan
AU  - Zhu G
AD  - Faculty of Genetics Program, Texas A&M University, 4467 TAMU, College Station, TX 
      77843-4467, USA.
AD  - Department of Veterinary Pathobiology, College of Veterinary Pathobiology, Texas 
      A&M University, 4467 TAMU, College Station, TX 77843-4467, USA.
LA  - eng
GR  - R01 AI044594/AI/NIAID NIH HHS/United States
GR  - R21 AI055278/AI/NIAID NIH HHS/United States
GR  - U01 AI046397/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Microbiology (Reading)
JT  - Microbiology (Reading, England)
JID - 9430468
RN  - 0 (RNA, Messenger)
RN  - 0 (Tubulin)
SB  - IM
MH  - Animals
MH  - Caco-2 Cells/parasitology
MH  - Cattle
MH  - Cattle Diseases/parasitology
MH  - Cell Line
MH  - Cryptosporidiosis/parasitology/veterinary
MH  - Cryptosporidium parvum/genetics/*growth & development/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Introns
MH  - *RNA Splicing
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Transcription, Genetic
MH  - Tubulin/*genetics/*metabolism
EDAT- 2004/05/11 05:00
MHDA- 2004/07/10 05:00
CRDT- 2004/05/11 05:00
PHST- 2004/05/11 05:00 [pubmed]
PHST- 2004/07/10 05:00 [medline]
PHST- 2004/05/11 05:00 [entrez]
AID - 10.1099/mic.0.26897-0 [doi]
PST - ppublish
SO  - Microbiology (Reading). 2004 May;150(Pt 5):1191-1195. doi: 10.1099/mic.0.26897-0.