PMID- 15133268
OWN - NLM
STAT- MEDLINE
DCOM- 20040628
LR  - 20190605
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 66
IP  - 4
DP  - 2004 Apr
TI  - Inhibitory effect of pyrimidine and purine nucleotides on the multiplication of 
      Babesia gibsoni: possible cause of low parasitemia and simultaneous 
      reticulocytosis in canine babesiosis.
PG  - 389-95
AB  - The present study was conducted to determine the cause of low parasitemia and 
      simultaneous reticulocytosis in canine babesiosis. The parasitemia was 
      significantly decreased in in vitro cultures of Babesia gibsoni by the 
      pretreatment of host canine erythrocytes with lead acetate, which is a specific 
      inhibitor of pyrimidine 5'-nucleotidase subclass I (P5N-I). The serum from dogs 
      chronically infected with B. gibsoni did not decrease the activities of 
      hexokinase, glucose-6-phosphate dehydrogenase or 6-phosphogluconate dehydrogenase 
      in canine reticulocytes, although it was previously reported that this serum had 
      inhibitory effects on both the maturation of reticulocytes and the canine P5N-I 
      and purine-specific 5'-nucleotidase activities. Furthermore, the in vitro 
      multiplication of B. gibsoni was significantly inhibited by pyrimidine 
      nucleotides such as cytidine 5'-monophosphate (5'-CMP), which is preferentially 
      catalyzed by P5N-I and also inhibits the morphological maturation of canine 
      reticulocytes. Purine nucleotides such as inosine 5'-monophosphate (5'-IMP) also 
      had an inhibitory effect on the multiplication of this parasite. These results 
      suggest that nucleotides such as 5'-CMP and 5'-IMP might accumulate in young 
      erythrocytes and/or serum in dogs infected with B. gibsoni as a result of the 
      decreased activity of erythrocyte 5'-nucleotidase, and the accumulation of these 
      nucleotides might inhibit the multiplication of this parasite and simultaneously 
      retard the maturation of reticulocytes. The results obtained from the in vitro 
      examinations in the present study may partially clarify the relationship between 
      low parasitemia and simultaneous reticulocytosis in vivo in canine babesiosis.
FAU - Hossain, Mohammad Alamgir
AU  - Hossain MA
AD  - Laboratory of Internal Medicine, Department of Veterinary Clinical Sciences, 
      Graduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
FAU - Yamato, Osamu
AU  - Yamato O
FAU - Yamasaki, Masahiro
AU  - Yamasaki M
FAU - Maede, Yoshimitsu
AU  - Maede Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Organometallic Compounds)
RN  - 131-99-7 (Inosine Monophosphate)
RN  - EC 3.1.3.5 (5'-Nucleotidase)
RN  - F469818O25 (Cytidine Monophosphate)
RN  - RX077P88RY (lead acetate)
SB  - IM
MH  - 5'-Nucleotidase/antagonists & inhibitors
MH  - Animals
MH  - Babesia/*drug effects/growth & development
MH  - Babesiosis/metabolism/*veterinary
MH  - Cytidine Monophosphate/metabolism/*pharmacology
MH  - Dog Diseases/metabolism/*parasitology
MH  - Dogs
MH  - Erythrocytes/metabolism/parasitology
MH  - Inosine Monophosphate/metabolism/*pharmacology
MH  - Organometallic Compounds/metabolism
MH  - Reticulocytosis/*drug effects
EDAT- 2004/05/11 05:00
MHDA- 2004/06/29 05:00
CRDT- 2004/05/11 05:00
PHST- 2004/05/11 05:00 [pubmed]
PHST- 2004/06/29 05:00 [medline]
PHST- 2004/05/11 05:00 [entrez]
AID - 10.1292/jvms.66.389 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2004 Apr;66(4):389-95. doi: 10.1292/jvms.66.389.