PMID- 15133645
OWN - NLM
STAT- MEDLINE
DCOM- 20040728
LR  - 20220331
IS  - 0093-7711 (Print)
IS  - 0093-7711 (Linking)
VI  - 56
IP  - 3
DP  - 2004 Jun
TI  - HLA-G and IL-10 in serum in relation to HLA-G genotype and polymorphisms.
PG  - 135-41
AB  - The expression and importance of the non-classical human leukocyte antigen (HLA) 
      class Ib gene, HLA-G, at the feto-maternal interface have been recognized. The 
      HLA-G molecule is almost monomorphic and expressed in both membrane-bound and 
      soluble isoforms. It has been shown to inhibit NK-mediated cell lysis and 
      influence cytokine expression. Recently, a possible boarder immunoregulatory 
      function of HLA-G also in adult life has been recognized. HLA-G gene polymorphism 
      has been linked to differences in gene expression profile of alternatively 
      spliced HLA-G transcripts and levels of specific HLA-G mRNA isoforms. On this 
      background it is of general interest to further elucidate any associations 
      between HLA-G polymorphism and protein expression. We have HLA-G genotyped 85 
      individuals attending IVF treatment, and further studied sHLA-G1/HLA-G5 and 
      interleukin-10 (IL-10) in serum samples. In 21% of the serum samples 
      sHLA-G1/HLA-G5 could be detected. There was no correlation between sHLA-G1/HLA-G5 
      and IL-10 concentrations in serum. Soluble HLA-G1/HLA-G5 was not detected in any 
      samples homozygous for a 14-bp insertion polymorphism in exon 8 of the 
      3'-untranslated region (3'UTR) of the HLA-G gene ( P=0.03; Fisher's exact test). 
      Polymorphisms in the 5'-upstream regulatory region (5'URR) of the HLA-G gene were 
      also studied. In conclusion, this study indicates that polymorphisms in the 3'UTR 
      and the 5'URR of the HLA-G gene may influence the expression of sHLA-G of 
      possible importance in pathological pregnancies and also in organ 
      transplantation.
FAU - Hviid, Thomas Vauvert F
AU  - Hviid TV
AD  - Department of Clinical Biochemistry, Copenhagen University Hospital, 
      Rigshospitalet, Denmark. hviid@dadlnet.dk
FAU - Rizzo, Roberta
AU  - Rizzo R
FAU - Christiansen, Ole B
AU  - Christiansen OB
FAU - Melchiorri, Loredana
AU  - Melchiorri L
FAU - Lindhard, Anette
AU  - Lindhard A
FAU - Baricordi, Olavio R
AU  - Baricordi OR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040507
PL  - United States
TA  - Immunogenetics
JT  - Immunogenetics
JID - 0420404
RN  - 0 (3' Untranslated Regions)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Exons
MH  - Female
MH  - Genotype
MH  - HLA Antigens/*blood/*genetics
MH  - HLA-G Antigens
MH  - Histocompatibility Antigens Class I/*blood/*genetics
MH  - Humans
MH  - Interleukin-10/*blood
MH  - Male
MH  - *Polymorphism, Genetic
MH  - Regulatory Sequences, Nucleic Acid
MH  - Sequence Deletion
EDAT- 2004/05/11 05:00
MHDA- 2004/07/29 05:00
CRDT- 2004/05/11 05:00
PHST- 2004/02/21 00:00 [received]
PHST- 2004/03/22 00:00 [accepted]
PHST- 2004/05/11 05:00 [pubmed]
PHST- 2004/07/29 05:00 [medline]
PHST- 2004/05/11 05:00 [entrez]
AID - 10.1007/s00251-004-0673-2 [doi]
PST - ppublish
SO  - Immunogenetics. 2004 Jun;56(3):135-41. doi: 10.1007/s00251-004-0673-2. Epub 2004 
      May 7.