PMID- 15134336
OWN - NLM
STAT- MEDLINE
DCOM- 20040621
LR  - 20061115
IS  - 1431-6730 (Print)
IS  - 1431-6730 (Linking)
VI  - 385
IP  - 3-4
DP  - 2004 Mar-Apr
TI  - Visualization of the three-dimensional organization of hypoxia-inducible factor-1 
      alpha and interacting cofactors in subnuclear structures.
PG  - 231-7
AB  - Cells need oxygen (O2) to meet their metabolic demands. Highly efficient systems 
      of O2-sensing have evolved to initiate responses enabling cells to adapt their 
      metabolism to reduced O2 availability. Of central importance is the activation of 
      hypoxia-inducible factor-1 (HIF-1), a transcription factor complex that controls 
      the expression of genes the products of which regulate glucose uptake and 
      metabolism, vasotonus and angiogenesis, oxygen capacity of the blood as well as 
      cell growth and death. Activation of HIF-1 requires the accumulation and nuclear 
      translocation of the HIF-1alpha subunit, its dimerization with HIF-1beta and the 
      binding of co-activator proteins such as p300. In this study we investigated the 
      three-dimensional (3D) distribution of HIF-1alpha within the nucleus and assigned 
      its localization to known nuclear compartments. Using two-photon microscopy we 
      determined the colocalization of HIF-1alpha and -beta subunits within nuclear 
      domains as well as overlaps between HIF-1alpha and p300. Our data provide 
      information on the nuclear distribution of HIF-1alpha with respect to subnuclear 
      domains that could serve as specific locations for hypoxia-induced gene 
      expression.
FAU - Berchner-Pfannschmidt, Utta
AU  - Berchner-Pfannschmidt U
AD  - Institut fur Physiologie, Universitat Duisburg-Essen, Hufelandstrasse 55, D-45122 
      Essen, Germany.
FAU - Wotzlaw, Christoph
AU  - Wotzlaw C
FAU - Merten, Evelyne
AU  - Merten E
FAU - Acker, Helmut
AU  - Acker H
FAU - Fandrey, Joachim
AU  - Fandrey J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Biol Chem
JT  - Biological chemistry
JID - 9700112
RN  - 0 (HIF1A protein, human)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Protein Subunits)
RN  - 0 (Transcription Factors)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Nucleus/*chemistry
MH  - Fluorescent Antibody Technique, Indirect
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Image Processing, Computer-Assisted/*methods
MH  - Imaging, Three-Dimensional/*methods
MH  - Microscopy, Fluorescence, Multiphoton/methods
MH  - Nuclear Proteins/analysis/chemistry
MH  - Protein Subunits/analysis/*chemistry
MH  - Transcription Factors/analysis/*chemistry
EDAT- 2004/05/12 05:00
MHDA- 2004/06/24 05:00
CRDT- 2004/05/12 05:00
PHST- 2004/05/12 05:00 [pubmed]
PHST- 2004/06/24 05:00 [medline]
PHST- 2004/05/12 05:00 [entrez]
AID - 10.1515/BC.2004.017 [doi]
PST - ppublish
SO  - Biol Chem. 2004 Mar-Apr;385(3-4):231-7. doi: 10.1515/BC.2004.017.