PMID- 15134888
OWN - NLM
STAT- MEDLINE
DCOM- 20040727
LR  - 20061115
IS  - 0167-7012 (Print)
IS  - 0167-7012 (Linking)
VI  - 57
IP  - 3
DP  - 2004 Jun
TI  - Universal primer PCR with DGGE for rapid detection of bacterial pathogens.
PG  - 409-13
AB  - A universal primer PCR (UPPCR) combined with denaturing gradient gel 
      electrophoresis (DGGE) was evaluated as a method permitting the rapid detection 
      of pathogens. The results show that this method is efficient at amplifying the 
      conserved regions of bacterial 16S rRNA genes with universal primers and can 
      detect causative bacterial pathogens rapidly. Six species of bacteria from 
      fisheries (Pseudomonas fluorescens, Vibrio anguillarum, Aeromonas hydrophila, 
      Vibrio fluvialis, Providencia rettgeri and Aeromonas sobria) were examined. Our 
      results indicate that the approach we undertook can be adopted not only for 
      axenic bacterial populations but also for mixed communities as well. Furthermore, 
      we were able to achieve the rapid detection of multiple bacteria a single in 
      sample. In addition, UPPCR-DGGE was shown to be better than previously reported 
      UPPCR-single-stranded conformation polymorphism (SSCP)-based methods for the 
      rapid detection of bacterial pathogens.
FAU - Ji, Niannian
AU  - Ji N
AD  - Department of Biology, School of Life Sciences, Xiamen University, Xiamen, Fujian 
      361005, PR China.
FAU - Peng, Bo
AU  - Peng B
FAU - Wang, Guizhong
AU  - Wang G
FAU - Wang, Sanying
AU  - Wang S
FAU - Peng, Xuanxian
AU  - Peng X
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - DNA Primers
MH  - DNA, Bacterial/chemistry/genetics
MH  - Electrophoresis, Polyacrylamide Gel/methods/*veterinary
MH  - Fish Diseases/*microbiology
MH  - Fishes
MH  - Gram-Negative Bacteria/genetics/*growth & development
MH  - Gram-Negative Bacterial Infections/diagnosis/microbiology/*veterinary
MH  - Polymerase Chain Reaction/*methods
MH  - Polymorphism, Single-Stranded Conformational
MH  - RNA, Ribosomal, 16S/chemistry/genetics
EDAT- 2004/05/12 05:00
MHDA- 2004/07/28 05:00
CRDT- 2004/05/12 05:00
PHST- 2003/07/15 00:00 [received]
PHST- 2004/02/05 00:00 [revised]
PHST- 2004/02/18 00:00 [accepted]
PHST- 2004/05/12 05:00 [pubmed]
PHST- 2004/07/28 05:00 [medline]
PHST- 2004/05/12 05:00 [entrez]
AID - S0167701204000557 [pii]
AID - 10.1016/j.mimet.2004.02.010 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2004 Jun;57(3):409-13. doi: 10.1016/j.mimet.2004.02.010.