PMID- 15134928
OWN - NLM
STAT- MEDLINE
DCOM- 20050318
LR  - 20141120
IS  - 0162-0134 (Print)
IS  - 0162-0134 (Linking)
VI  - 98
IP  - 5
DP  - 2004 May
TI  - Resonance Raman study on synergistic activation of soluble guanylate cyclase by 
      imidazole, YC-1 and GTP.
PG  - 824-32
AB  - Soluble guanylate cyclase (sGC), a physiological nitric oxide (NO) receptor, is a 
      heme-containing protein and catalyzes the conversion of GTP to cyclic GMP. We 
      found that 200 mM imidazole moderately activated sGC in the coexistence with 
      3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole (YC-1), although imidazole or YC-1 
      alone had little effect for activation. GTP facilitated this process. Resonance 
      Raman spectra of imidazole complex of native sGC and CO-bound sGC (CO-sGC) have 
      demonstrated that a simple heme adduct with imidazole at the sixth coordination 
      position is not present for both sGC and CO-sGC below 200 mM of the imidazole 
      concentration and that the Fe-CO stretching band (nuFe-CO)) appears at 492 cm(-1) 
      in the presence of imidazole compared with 473 cm(-1) in its absence. Both 
      frequencies fall on the line of His-coordinated heme proteins in the nuFe-CO vs 
      nuC-O plot. However, it is stressed that the CO-heme of sGC becomes apparently 
      photo-inert in a spinning cell in the presence of imidazole, suggesting the 
      formation of five-coordinate CO-heme or of six-coordinate heme with a very weak 
      trans ligand. These observations suggest that imidazole alters not only the 
      polarity of heme pocket but also the coordination structure at the fifth 
      coordination side presumably by perturbing the heme-protein interactions at 
      propionic side chains. Despite the fact that the isolated sGC stays in the 
      reduced state and is not oxidized by O(2), sGC under the high concentration of 
      imidazole (1.2 M) yielded nu4 at 1373 cm(-1) even after its removal by 
      gel-filtration, but addition of dithionite gave the strong nu4 band at 1360 
      cm(-1). This indicated that imidazole caused autoxidation of sGC.
FAU - Pal, Biswajit
AU  - Pal B
AD  - Center for Integrative Bioscience, Okazaki National Research Institutes, 
      Higashiyama 5-1, Myodaiji, Okazaki 444-8585, Japan.
FAU - Li, Zhengqiang
AU  - Li Z
FAU - Ohta, Takehiro
AU  - Ohta T
FAU - Takenaka, Shigeo
AU  - Takenaka S
FAU - Tsuyama, Shingo
AU  - Tsuyama S
FAU - Kitagawa, Teizo
AU  - Kitagawa T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Inorg Biochem
JT  - Journal of inorganic biochemistry
JID - 7905788
RN  - 0 (Imidazoles)
RN  - 0 (Indazoles)
RN  - 154453-18-6 (3-(5'-hydroxymethyl-2'-furyl)-1-benzylindazole)
RN  - 42VZT0U6YR (Heme)
RN  - 7GBN705NH1 (imidazole)
RN  - 86-01-1 (Guanosine Triphosphate)
RN  - EC 4.6.1.2 (Guanylate Cyclase)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Drug Synergism
MH  - Enzyme Activation/drug effects
MH  - Guanosine Triphosphate/administration & dosage/metabolism/*pharmacology
MH  - Guanylate Cyclase/*chemistry/*metabolism
MH  - Heme/chemistry
MH  - Imidazoles/administration & dosage/*pharmacology
MH  - In Vitro Techniques
MH  - Indazoles/administration & dosage/*pharmacology
MH  - Solubility
MH  - Spectrum Analysis, Raman
EDAT- 2004/05/12 05:00
MHDA- 2005/03/19 09:00
CRDT- 2004/05/12 05:00
PHST- 2003/10/02 00:00 [received]
PHST- 2003/12/03 00:00 [revised]
PHST- 2003/12/08 00:00 [accepted]
PHST- 2004/05/12 05:00 [pubmed]
PHST- 2005/03/19 09:00 [medline]
PHST- 2004/05/12 05:00 [entrez]
AID - S0162013403004574 [pii]
AID - 10.1016/j.jinorgbio.2003.12.007 [doi]
PST - ppublish
SO  - J Inorg Biochem. 2004 May;98(5):824-32. doi: 10.1016/j.jinorgbio.2003.12.007.