PMID- 15135098
OWN - NLM
STAT- MEDLINE
DCOM- 20050121
LR  - 20161124
IS  - 1570-0232 (Print)
IS  - 1570-0232 (Linking)
VI  - 805
IP  - 2
DP  - 2004 Jun 15
TI  - Separation and determination of dexamethasone sodium phosphate in cochlear 
      perilymph fluid by liquid chromatography with ultraviolet monitoring and 
      electrospray ionization mass spectrometry characterization.
PG  - 255-60
AB  - The method for separation and determination of dexamethasone sodium phosphate 
      (DexP) in cochlear perilymph fluid (CPF) of cavy was developed using HPLC with 
      ultraviolet (UV) monitoring and electrospray ionization/mass spectrometry 
      (ESI/MS) identification. The quantitative determination of DexP in CPF was 
      achieved by HPLC with UV detection at 245 nm. The separation was carried out on a 
      Phenomenex ODS(3) column ( 250 mm x 4.6 mm i.d., 5 microm) with the mobile phase 
      of acetonitrile-5mmol/l ammonium acetate (23:77 (v/v)) at a flow rate of 1.0 
      ml/min. DexP was baseline separated from the matrices of CPF blanks within 15 
      min. The linearity ranged from 0.5 to 50 microg/ml. The limit of detection was 
      0.10 microg/ml. The recovery ranged from 98.5 to 100.8%. The relative standard 
      deviations (R.S.D.s) of intra- and inter-day peak area were between 0.7-1.3 and 
      1.2-3.5%, respectively. Both full scan MS and MS2 of DexP with positive and 
      negative polarity were obtained and elucidated. The specific ions were chosen to 
      characterize DexP in the CPF sample. Using the proposed HPLC-UV-ESI/MS method, 
      the concentration of DexP in CPF samples after both vein and middle ear 
      injections were determined, and the relationships between concentration and time 
      were obtained. This method offered reference data for clinical investigation of 
      DexP to cure ear diseases.
FAU - Liu, Hongxia
AU  - Liu H
AD  - Department of Chemistry, The Key Laboratory of Chemical Biology and Organic 
      Chemistry of Henan, Zhengzhou University, 450052, PR China. liuhongxia999@371.net
FAU - Chen, Xiaolan
AU  - Chen X
FAU - Zhang, Shusheng
AU  - Zhang S
FAU - Qu, Lingbo
AU  - Qu L
FAU - Zhao, Yufen
AU  - Zhao Y
FAU - Liu, Hongjian
AU  - Liu H
FAU - Dong, Mingmin
AU  - Dong M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 2BP70L44PR (dexamethasone 21-phosphate)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Cochlea/*chemistry
MH  - Dexamethasone/*analogs & derivatives/*analysis
MH  - Humans
MH  - Perilymph/*chemistry
MH  - Reproducibility of Results
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
EDAT- 2004/05/12 05:00
MHDA- 2005/01/22 09:00
CRDT- 2004/05/12 05:00
PHST- 2003/10/15 00:00 [received]
PHST- 2004/02/28 00:00 [revised]
PHST- 2004/03/04 00:00 [accepted]
PHST- 2004/05/12 05:00 [pubmed]
PHST- 2005/01/22 09:00 [medline]
PHST- 2004/05/12 05:00 [entrez]
AID - S1570023204002132 [pii]
AID - 10.1016/j.jchromb.2004.03.005 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2004 Jun 15;805(2):255-60. doi: 
      10.1016/j.jchromb.2004.03.005.