PMID- 15136157
OWN - NLM
STAT- MEDLINE
DCOM- 20040804
LR  - 20161126
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1656
IP  - 1
DP  - 2004 May 12
TI  - Structural characterization of a binuclear center of a Cu-containing NO reductase 
      homologue from Roseobacter denitrificans: EPR and resonance Raman studies.
PG  - 37-45
AB  - Aerobic phototrophic bacterium Roseobacter denitrificans has a nitric oxide 
      reductase (NOR) homologue with cytochrome c oxidase (CcO) activity. It is 
      composed of two subunits that are homologous with NorC and NorB, and contains 
      heme c, heme b, and copper in a 1:2:1 stoichiometry. This enzyme has virtually no 
      NOR activity. Electron paramagnetic resonance (EPR) spectra of the air-oxidized 
      enzyme showed signals of two low-spin hemes at 15 K. The high-spin heme species 
      having relatively low signal intensity indicated that major part of heme b3 is 
      EPR-silent due to an antiferromagnetic coupling to an adjacent CuB forming a 
      Fe-Cu binuclear center. Resonance Raman (RR) spectrum of the oxidized enzyme 
      suggested that heme b3 is six-coordinate high-spin species and the other hemes 
      are six-coordinate low-spin species. The RR spectrum of the reduced enzyme showed 
      that all the ferrous hemes are six-coordinate low-spin species. Nu(Fe-CO) and 
      nu(C-O) stretching modes were observed at 523 and 1969 cm(-1), respectively, for 
      CO-bound enzyme. In spite of the similarity to NOR in the primary structure, the 
      frequency of nu(Fe-CO) mode is close to those of aa3- and bo3-type oxidases 
      rather than that of NOR.
CI  - Copyright 2004 Elsevier B.V.
FAU - Matsuda, Yuji
AU  - Matsuda Y
AD  - Department of Biology, Graduate School of Sciences, Kyushu University, 6-10-1 
      Hakozaki, Higashi, Fukuoka 812-8581, Japan.
FAU - Uchida, Takeshi
AU  - Uchida T
FAU - Hori, Hiroshi
AU  - Hori H
FAU - Kitagawa, Teizo
AU  - Kitagawa T
FAU - Arata, Hiroyuki
AU  - Arata H
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 789U1901C5 (Copper)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.7.2.5 (nitric-oxide reductase)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Copper/*metabolism
MH  - Electron Spin Resonance Spectroscopy
MH  - Electron Transport Complex IV/chemistry
MH  - Optics and Photonics
MH  - Oxidoreductases/*chemistry/metabolism
MH  - Roseobacter/*enzymology
MH  - Spectrum Analysis, Raman
MH  - Structural Homology, Protein
EDAT- 2004/05/12 05:00
MHDA- 2004/08/05 05:00
CRDT- 2004/05/12 05:00
PHST- 2003/09/03 00:00 [received]
PHST- 2003/12/19 00:00 [revised]
PHST- 2004/01/07 00:00 [accepted]
PHST- 2004/05/12 05:00 [pubmed]
PHST- 2004/08/05 05:00 [medline]
PHST- 2004/05/12 05:00 [entrez]
AID - S0005272804000192 [pii]
AID - 10.1016/j.bbabio.2004.01.004 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2004 May 12;1656(1):37-45. doi: 
      10.1016/j.bbabio.2004.01.004.