PMID- 15136507
OWN - NLM
STAT- MEDLINE
DCOM- 20041209
LR  - 20220310
IS  - 1524-4539 (Electronic)
IS  - 0009-7322 (Linking)
VI  - 109
IP  - 21
DP  - 2004 Jun 1
TI  - C-reactive protein promotes monocyte chemoattractant protein-1--mediated 
      chemotaxis through upregulating CC chemokine receptor 2 expression in human 
      monocytes.
PG  - 2566-71
AB  - BACKGROUND: Inflammation plays a crucial role in atherosclerosis. An elevated 
      serum C-reactive protein (CRP) level is a strong marker for future 
      atherosclerotic cardiovascular diseases. In addition, recent data suggest that 
      CRP may directly promote atherogenesis. In this study, we investigated whether 
      CRP can directly activate human circulating monocytes. METHODS AND RESULTS: 
      Incubation of THP-1 monocytes with CRP (10 microg/mL) increased CC chemokine 
      receptor 2 (CCR2) expression at both the protein and transcript levels, which in 
      turn enhanced chemotaxis mediated by monocyte chemoattractant protein-1 (MCP-1) 
      up to 2-fold. The CRP-induced upregulation of CCR2 expression involved binding of 
      CRP to the FcgammaR, most notably FcgammaRI, and phospholipase D1 activation. 
      Serum high-sensitivity CRP levels in 52 normocholesterolemic human subjects were 
      positively correlated with CCR2 surface expression on circulating monocytes 
      (r=0.62, P<0.001) and MCP-1-mediated monocyte chemotaxis (r=0.53, P<0.001). 
      CONCLUSIONS: Elevated blood CRP levels may promote accumulation of monocytes in 
      the atherogenic arterial wall by increasing chemotactic activities of monocytes 
      in response to MCP-1.
FAU - Han, Ki Hoon
AU  - Han KH
AD  - Asan Medical Center, University of Ulsan College of Medicine, Seoul, South Korea. 
      steadyhan@amc.seoul.kr
FAU - Hong, Kyung-Hee
AU  - Hong KH
FAU - Park, Jae-Hyeong
AU  - Park JH
FAU - Ko, Jesang
AU  - Ko J
FAU - Kang, Duk-Hyun
AU  - Kang DH
FAU - Choi, Kee-Joon
AU  - Choi KJ
FAU - Hong, Myeong-Ki
AU  - Hong MK
FAU - Park, Seong-Wook
AU  - Park SW
FAU - Park, Seung-Jung
AU  - Park SJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20040510
PL  - United States
TA  - Circulation
JT  - Circulation
JID - 0147763
RN  - 0 (CCL2 protein, human)
RN  - 0 (CCR2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (FCGR1A protein, human)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Receptors, IgG)
RN  - 0 (Recombinant Proteins)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 3.1.4.4 (Phospholipase D)
RN  - EC 3.1.4.4 (phospholipase D1)
SB  - IM
MH  - Arteriosclerosis/blood/*physiopathology
MH  - C-Reactive Protein/*pharmacology
MH  - Cells, Cultured/drug effects
MH  - Chemokine CCL2/*physiology
MH  - Chemotaxis/*drug effects/physiology
MH  - Cholesterol/blood
MH  - Enzyme Activation
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Monocytes/*drug effects/physiology
MH  - Muscle, Smooth, Vascular/cytology
MH  - Myocytes, Smooth Muscle/cytology/drug effects
MH  - Phospholipase D/metabolism
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/*biosynthesis/genetics
MH  - Receptors, IgG/drug effects/physiology
MH  - Recombinant Proteins/pharmacology
MH  - Transcription, Genetic/drug effects
MH  - Up-Regulation/drug effects
EDAT- 2004/05/12 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/05/12 05:00
PHST- 2004/05/12 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/05/12 05:00 [entrez]
AID - 01.CIR.0000131160.94926.6E [pii]
AID - 10.1161/01.CIR.0000131160.94926.6E [doi]
PST - ppublish
SO  - Circulation. 2004 Jun 1;109(21):2566-71. doi: 10.1161/01.CIR.0000131160.94926.6E. 
      Epub 2004 May 10.