PMID- 15137815
OWN - NLM
STAT- MEDLINE
DCOM- 20040707
LR  - 20131121
IS  - 0021-8561 (Print)
IS  - 0021-8561 (Linking)
VI  - 52
IP  - 10
DP  - 2004 May 19
TI  - Determination of beta-lactams in milk using a surface plasmon resonance-based 
      biosensor.
PG  - 2791-6
AB  - Two surface plasmon resonance (SPR)-based biosensor assays for detection of 
      beta-lactam antibiotics in milk are reported. The assays are based on the 
      enzymatic activity of a carboxypeptidase converting a 3-peptide into a 2-peptide, 
      a reaction that is inhibited in the presence of beta-lactams. Antibodies were 
      used to measure either the amount of formed enzymatic product or the amount of 
      remaining enzymatic substrate. Both assays detected different beta-lactams at or 
      below European maximum residue limits (MRLs), and the detection limit for 
      penicillin G was 1.2 microg/kg and 1.5 microg/kg for the 2- and 3-peptide assays, 
      respectively. The precision (CV) was < 5%, both within and between assays at the 
      penicillin G MRL (4 microg/kg). The biosensor results obtained upon analysis of 
      incurred milk samples were compared with results obtained by liquid 
      chromatography (HPLC), and the method agreements were, in general, good.
FAU - Gustavsson, Eva
AU  - Gustavsson E
AD  - Department of Food Science, Swedish University of Agricultural Sciences, P.O. Box 
      7051, 750 07 Uppsala, Sweden.
FAU - Degelaen, Jacques
AU  - Degelaen J
FAU - Bjurling, Peter
AU  - Bjurling P
FAU - Sternesjo, Ase
AU  - Sternesjo A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (beta-Lactams)
RN  - EC 3.4.- (Carboxypeptidases)
RN  - Q42T66VG0C (Penicillin G)
SB  - IM
MH  - Animals
MH  - Carboxypeptidases
MH  - Chromatography, High Pressure Liquid
MH  - Milk/*chemistry
MH  - Penicillin G/analysis
MH  - Sensitivity and Specificity
MH  - *Surface Plasmon Resonance
MH  - beta-Lactams/*analysis
EDAT- 2004/05/13 05:00
MHDA- 2004/07/09 05:00
CRDT- 2004/05/13 05:00
PHST- 2004/05/13 05:00 [pubmed]
PHST- 2004/07/09 05:00 [medline]
PHST- 2004/05/13 05:00 [entrez]
AID - 10.1021/jf0344284 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2004 May 19;52(10):2791-6. doi: 10.1021/jf0344284.