PMID- 15138116
OWN - NLM
STAT- MEDLINE
DCOM- 20040715
LR  - 20041117
IS  - 1499-3872 (Print)
VI  - 3
IP  - 2
DP  - 2004 May
TI  - Effect of IL-18 on peripheral blood mononuclear cells of chronic hepatitis B and 
      hepatitis B virus DNA released by HepG2.2.15 cell lines.
PG  - 230-4
AB  - BACKGROUND: Interleukin-18 (IL-18), a pro-inflammatory cytokine that induces 
      interferon-gamma (IFN-gamma) production in T cells and natural killer cells, 
      plays a critical role in the T-lymphocyte helper type 1 (Th1) response. This 
      study was designed to explore the effect of IL-18 on peripheral blood mononuclear 
      cells (PBMCs) derived from chronic hepatitis B (CHB) and on hepatitis B virus 
      (HBV) DNA released by HepG2.2.15 cell lines, which were transfected with 
      hepatitis B virus gene in vitro. METHODS: PBMCs isolated from 25 healthy people 
      and 25 patients with CHB were stimulated with HBcAg and IL-18 of various 
      concentrations for 72 hours. The levels of IFN-gamma in the supernatants of 
      cultured PBMCs were determined by ELISA. After the stimulation of IL-18 of 
      various concentrations, PBMCs derived from one patient were co-cultured for 96 
      hours with HepG2.2.15 cells which had been cultured for 24 hours, and then the 
      supernatants were collected by centrifugation and used for HBV DNA quantitative 
      assay. RESULTS: When PBMCs were stimulated by HBcAg and IL-18 at various 
      concentrations, the levels of IFN-gamma in the supernatants of CHB groups were 
      much higher than those in normal control groups, at 0.2 ng/ml: t=11.70, P<0.01; 
      at 1.0 ng/ml: t=16.19, P<0.01; and at 5.0 ng/ml: t=20.12, P<0.01. In the CHB 
      groups, the levels of IFN-gamma in the supernatants of PBMCs stimulated by HBcAg 
      alone were lower than both those stimulated by HBcAg and IL-18 at various 
      concentrations and those stimulated by HBcAg and IL-18 (5.0 ng/ml) together with 
      IL-12 (mild: t=2.20, P<0.05; moderate: t=2.97, P<0.05; severe: t=0.66, P>0.05). 
      The content of HBV DNA in the supernatant of co-cultivation of HepG2.2.15 cells 
      and PBMCs without stimulated materials was higher than that stimulated by HBcAg 
      and IL-18 at various concentrations of HBcAg and IL-18 together with 
      IL-12/IFN-alpha 1b. CONCLUSION: IL-18 can induce IFN-gamma secretion and probably 
      play a key role in the modulation of both innate and adaptive immunity. It has 
      implications in improving immunoregulatory effect and increasing the ability of 
      immune cells to kill cells infected by virus.
FAU - Sun, Ying
AU  - Sun Y
AD  - Department of Infectious Diseases, First Clinical College, Harbin Medical 
      University, Harbin 150001, China.
FAU - Chen, Huan-Yong
AU  - Chen HY
FAU - Xin, Shao-Jie
AU  - Xin SJ
LA  - eng
PT  - Journal Article
PL  - Singapore
TA  - Hepatobiliary Pancreat Dis Int
JT  - Hepatobiliary & pancreatic diseases international : HBPD INT
JID - 101151457
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (DNA, Viral)
RN  - 0 (Interleukin-18)
SB  - IM
MH  - Adjuvants, Immunologic/*pharmacology
MH  - Adolescent
MH  - Adult
MH  - Cell Line
MH  - DNA, Viral/*drug effects/immunology
MH  - Female
MH  - Hepatitis B virus/*drug effects/genetics/immunology
MH  - Hepatitis B, Chronic/*immunology
MH  - Humans
MH  - Interleukin-18/immunology/*pharmacology
MH  - Leukocytes, Mononuclear/*drug effects/immunology
MH  - Male
MH  - Middle Aged
MH  - Transfection
EDAT- 2004/05/13 05:00
MHDA- 2004/07/16 05:00
CRDT- 2004/05/13 05:00
PHST- 2004/05/13 05:00 [pubmed]
PHST- 2004/07/16 05:00 [medline]
PHST- 2004/05/13 05:00 [entrez]
AID - 221 [pii]
PST - ppublish
SO  - Hepatobiliary Pancreat Dis Int. 2004 May;3(2):230-4.