PMID- 15138130
OWN - NLM
STAT- MEDLINE
DCOM- 20040715
LR  - 20181130
IS  - 1499-3872 (Print)
VI  - 3
IP  - 2
DP  - 2004 May
TI  - Clinical relationship between MDR1 gene and gallbladder cancer.
PG  - 296-9
AB  - BACKGROUND: The most common mechanisms of multidrug resistance (MDR) in cancer 
      cells is the expression of an energy-dependent exfflux pump. P-glycoprotein 
      (P-gp) encoded by MDR1 gene and multidrug associated protein (MRP) are well known 
      proteins associated with MDR. In human cancers, the MDR1 gene expression is 
      common in patients with intrinsic and acquired MDR. It is a major therapeutic 
      problem in cancer chemotherapy. Previously we found that the MDR of HCC is 
      related to MRP gene expression and initiates the intrinsic MDR. The aim of this 
      study is to study the expression of MDR1 gene encoding P-gp and MDRl mRNA in 
      primary gallbladder carcinoma, and analyze its clinical significance. METHODS: 
      Immunohistochemistry (IHC) S-P method and in situ polymerase chain reaction 
      (ISPCR) were used to detect the expression of P-gp and MDR1 mRNA in 53 cases of 
      untreated primary gallbladder carcinoma and 12 cases of cholecystitis (archival 
      paraffin-embedded tissues). RESULTS: The positive expression rates of P-gp and 
      MDR1 mRNA in the 53 cases and 12 cases were 60.38%, 71.69% and 25.00%, 33.33%, 
      respectively. There was a significant difference between the two groups (P<0.05). 
      The positive expression rate of P-gp and MDR1mRNA were 69.44%, 83.33% and 41.18%, 
      47.06% respectively in tissues in stage of Nevin I-III against Nevin IV, V 
      (P<0.05). In well, moderately differentiated gallbladder carcinoma tissues, their 
      expressions were 79.49%, 69.23% against 50.00%, 35.71% in low, undifferentiated 
      tissues (P<0.05). CONCLUSIONS: MDR to gallbladder carcinoma is closely related to 
      the intrinsic MDR and it provides an important evidence to reverse the MDR by 
      detection of the MDR1 gene. Meanwhile, MDR1 gene expression in gallbladder 
      carcinoma is correlated with some biological characteristics, takes part in the 
      carcinogenesis of gallbladder tissues, and acts as a valuable biomarker of 
      prognosis.
FAU - Wang, Bai-Lin
AU  - Wang BL
AD  - Third Department of Surgery, First Affiliated Hospital of Guangzhou University of 
      Traditional Chinese Medicine, Guangzhou 510405, China. wbl672000@yahoo.com.cn
FAU - Zhai, Hai-Ying
AU  - Zhai HY
FAU - Chen, Bing-Yi
AU  - Chen BY
FAU - Zhai, Shu-Ping
AU  - Zhai SP
FAU - Yang, Hai-Yan
AU  - Yang HY
FAU - Chen, Xiao-Ping
AU  - Chen XP
FAU - Zhao, Wen-Tao
AU  - Zhao WT
FAU - Meng, Lei
AU  - Meng L
LA  - eng
PT  - Journal Article
PL  - Singapore
TA  - Hepatobiliary Pancreat Dis Int
JT  - Hepatobiliary & pancreatic diseases international : HBPD INT
JID - 101151457
RN  - 0 (ATP Binding Cassette Transporter, Subfamily B, Member 1)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily B, Member 1/biosynthesis/*genetics
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/*genetics
MH  - Carcinoma/*genetics/metabolism
MH  - Cell Transformation, Neoplastic/genetics
MH  - Drug Resistance, Neoplasm/genetics
MH  - Female
MH  - Gallbladder Neoplasms/*genetics/metabolism
MH  - Genes, MDR/*genetics
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Prognosis
MH  - RNA, Messenger/genetics
EDAT- 2004/05/13 05:00
MHDA- 2004/07/16 05:00
CRDT- 2004/05/13 05:00
PHST- 2004/05/13 05:00 [pubmed]
PHST- 2004/07/16 05:00 [medline]
PHST- 2004/05/13 05:00 [entrez]
AID - 235 [pii]
PST - ppublish
SO  - Hepatobiliary Pancreat Dis Int. 2004 May;3(2):296-9.