PMID- 15140027
OWN - NLM
STAT- MEDLINE
DCOM- 20050113
LR  - 20040513
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 63
IP  - 6
DP  - 2004 Jun
TI  - Multiplexed analysis of polymorphisms in the HLA gene complex using bead array 
      chips.
PG  - 518-28
AB  - A novel custom bead array technology is introduced, and it is applied to 
      multiplexed analysis of highly polymorphic human leukocyte antigen (HLA) genetic 
      loci. Our technology combines the construction of probe libraries on 
      color-encoded microparticles (beads) with semiconductor chip processing to 
      produce custom-designed high-density bead array chips in large quantities. Using 
      this novel assay format, two modes of parallel molecular typing of the HLA 
      complex were implemented, namely direct hybridization, illustrated here for class 
      II HLA-DR, and a novel format of on-chip polymerase-mediated primer elongation, 
      illustrated here for class I HLA-A, HLA-B, and HLA-DR using patient and reference 
      cell-line DNA samples. Hybridization-mediated multiplexed analysis of 
      polymorphism method was validated with 142 samples, resulting in 100% concordance 
      with sequence-specific oligonucleotide typing results and a concomitant average 
      of 40% less allele ambiguity. In addition, elongation and hybridization reactions 
      were combined to identify multiple polymorphisms on the same phase of DNA for 
      allele identification.
CI  - Copyright 2004 Blackwell Munksgaard
FAU - Li, A X
AU  - Li AX
AD  - BioArray Solutions Ltd, Warren, NJ, USA.
FAU - Seul, M
AU  - Seul M
FAU - Cicciarelli, J
AU  - Cicciarelli J
FAU - Yang, J C
AU  - Yang JC
FAU - Iwaki, Y
AU  - Iwaki Y
LA  - eng
PT  - Journal Article
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA Antigens)
SB  - IM
MH  - HLA Antigens/*genetics
MH  - Humans
MH  - *Microspheres
MH  - *Oligonucleotide Array Sequence Analysis
MH  - *Polymorphism, Genetic
EDAT- 2004/05/14 05:00
MHDA- 2005/01/14 09:00
CRDT- 2004/05/14 05:00
PHST- 2004/05/14 05:00 [pubmed]
PHST- 2005/01/14 09:00 [medline]
PHST- 2004/05/14 05:00 [entrez]
AID - TAN219 [pii]
AID - 10.1111/j.0001-2815.2004.00219.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2004 Jun;63(6):518-28. doi: 10.1111/j.0001-2815.2004.00219.x.