PMID- 15140218
OWN - NLM
STAT- MEDLINE
DCOM- 20040625
LR  - 20171116
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 122
IP  - 5
DP  - 2004 May
TI  - Characterization of the sensitizing potential of chemicals by in vitro analysis 
      of dendritic cell activation and skin penetration.
PG  - 1154-64
AB  - Development of in vitro models to identify sensitizing chemicals receives public 
      interest since animal testing should be avoided whenever possible. In this 
      article we analyze two essential properties of sensitizing chemicals: skin 
      penetration and dendritic cell (DC) activation. Activation of immature DC derived 
      from peripheral blood monocytes was evaluated by flow cytometric analysis of CD86 
      positive cells and quantitative measurement of interleukin-1beta and aquaporin P3 
      gene expression. The sensitizer 2,4,6-trinitrobenzenesulfonic acid induced a 
      concentration-dependent response for all parameters, whereas the irritant sodium 
      lauryl sulfate did not. When two related aromatic amines, p-toluylenediamine 
      (PTD) and hydroxyethyl-p-phenylenediamine (HE-PPD) were tested, both induced 
      substantial DC activation indicating their potential sensitizing properties. 
      These findings contrasted with in vivo results: in murine local lymph node assays 
      (LLNA) PTD, but not HE-PPD, was sensitizing using acetone/aqua/olive oil as 
      vehicle. Skin penetration measurement revealed that this was due to 
      bioavailability differences. On retesting HE-PPD in the LLNA using the 
      penetration enhancer dimethylsulfoxide as vehicle, it induced a specific 
      response. We conclude that in vitro analysis of DC activation capability of the 
      two selected chemicals demonstrates that prediction of skin sensitization 
      potential is possible provided that skin penetration data indicate sufficient 
      bioavailability of the test compound.
FAU - Aeby, Pierre
AU  - Aeby P
AD  - Cosmital SA, (Research company of Wella AG) Marly, Switzerland. paeby@cosmital.ch
FAU - Wyss, Christoph
AU  - Wyss C
FAU - Beck, Heinz
AU  - Beck H
FAU - Griem, Peter
AU  - Griem P
FAU - Scheffler, Heike
AU  - Scheffler H
FAU - Goebel, Carsten
AU  - Goebel C
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (AQP3 protein, human)
RN  - 0 (Amines)
RN  - 0 (Antigens, CD)
RN  - 0 (Aqp3 protein, mouse)
RN  - 0 (Aquaporins)
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD86 protein, human)
RN  - 0 (Cd86 protein, mouse)
RN  - 0 (Interleukin-1)
RN  - 0 (Irritants)
RN  - 0 (Membrane Glycoproteins)
RN  - 158801-98-0 (Aquaporin 3)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Amines/pharmacology
MH  - Animal Testing Alternatives
MH  - Animals
MH  - Antigens, CD/analysis
MH  - Aquaporin 3
MH  - Aquaporins/genetics
MH  - B7-2 Antigen
MH  - Cell Differentiation/drug effects/immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/*drug effects/*immunology
MH  - Dermatitis, Allergic Contact/*immunology
MH  - Female
MH  - Gene Expression/drug effects
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/pharmacology
MH  - Humans
MH  - In Vitro Techniques
MH  - Interleukin-1/genetics
MH  - Interleukin-4/pharmacology
MH  - Irritants/*pharmacology
MH  - Membrane Glycoproteins/analysis
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Monocytes/chemistry/cytology/immunology
MH  - Skin/*immunology
MH  - Swine
EDAT- 2004/05/14 05:00
MHDA- 2004/06/26 05:00
CRDT- 2004/05/14 05:00
PHST- 2004/05/14 05:00 [pubmed]
PHST- 2004/06/26 05:00 [medline]
PHST- 2004/05/14 05:00 [entrez]
AID - S0022-202X(15)30802-2 [pii]
AID - 10.1111/j.0022-202X.2004.22402.x [doi]
PST - ppublish
SO  - J Invest Dermatol. 2004 May;122(5):1154-64. doi: 
      10.1111/j.0022-202X.2004.22402.x.