PMID- 15140254
OWN - NLM
STAT- MEDLINE
DCOM- 20050726
LR  - 20181113
IS  - 1477-7827 (Electronic)
IS  - 1477-7827 (Linking)
VI  - 2
DP  - 2004 May 12
TI  - Luteal 3beta-hydroxysteroid dehydrogenase and 20alpha-hydroxysteroid 
      dehydrogenase activities in the rat corpus luteum of pseudopregnancy: effect of 
      the deciduoma reaction.
PG  - 22
AB  - BACKGROUND: In the rat, the maintenance of gestation is dependent on progesterone 
      production from the corpora lutea (CL), which are under the control of pituitary, 
      decidual and placental hormones. The luteal metabolism of progesterone during 
      gestation has been amply studied. However, the regulation of progesterone 
      synthesis and degradation during pseudopregnancy (PSP), in which the CL are 
      mainly under the control of pituitary prolactin (PRL), is not well known. The 
      objectives of this investigation were: i) to study the luteal metabolism of 
      progesterone during PSP by measuring the activities of the enzymes 
      3beta-hydroxysteroid dehydrogenase (3betaHSD), involved in progesterone 
      biosynthesis, and that of 20alpha-hydroxysteroid dehydrogenase (20alphaHSD), 
      involved in progesterone catabolism; and ii) to determine the role of 
      decidualization on progesterone metabolism in PSP. METHODS: PSP was induced 
      mechanically at 10:00 h on the estrus of 4-day cycling Wistar rats, and the 
      stimulus for decidualization was provided by scratching the uterus on day 4 of 
      PSP. 3betaHSD and 20alphaHSD activities were measured in the CL isolated from 
      ovaries of PSP rats using a spectrophotometric method. Serum concentrations of 
      progesterone, PRL, androstenedione, and estradiol were measured by 
      radioimmunoassay (RIA). RESULTS: The PSP stage induced mechanically in cycling 
      rats lasted 11.3 +/- 0.09 days (n = 14). Serum progesterone concentration was 
      high until day 10 of PSP, and declined thereafter. Serum PRL concentration was 
      high on the first days of PSP but decreased significantly from days 6 to 9, 
      having minimal values on days 10 and 11. Luteal 3betaHSD activities were elevated 
      until day 6 of PSP, after which they progressively declined, reaching minimal 
      values at the end of PSP. Luteal 20alphaHSD activities were very low until day 9, 
      but abruptly increased at the end of PSP. When the deciduoma was induced by 
      scratching the uterus of pseudopregnant animals on day 4 (PSP+D), PSP was 
      extended to 18 +/- 2.2 days (n = 8). In PSP + D rats, serum progesterone and PRL 
      levels, and luteal 3betaHSD activities were higher than in pseudopregnant rats on 
      day 11. Decidualization also prevented the increase in luteal 20alphaHSD 
      activities observed on day 11 of PSP. Administration of the dopaminergic agonist 
      CB154 in PSP + D rats on day 10 of PSP induced a decline in both serum PRL and 
      progesterone on day 11 of PSP, values that were not different from that of 
      pseudopregnant controls. CONCLUSIONS: We have established that during the final 
      period of PSP a decline in progesterone biosynthesis occurs before the increase 
      in progesterone catabolism. We have also shown that decidualization in 
      pseudopregnant rats extends the life of the CL by prolonging the production of 
      pituitary PRL, and by maintaining high 3betaHSD and low 20alphaHSD activities 
      within the CL leading to sustained production of progesterone.
FAU - Clementi, Marisa A
AU  - Clementi MA
AD  - Laboratorio de Reproduccion y Lactancia, IMBECU-CONICET, Mendoza, Argentina. 
      mclementi@med.uchile.cl
FAU - Deis, Ricardo P
AU  - Deis RP
FAU - Telleria, Carlos M
AU  - Telleria CM
LA  - eng
GR  - P20 RR016479/RR/NCRR NIH HHS/United States
GR  - 5 P20 RR16479-02/RR/NCRR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20040512
PL  - England
TA  - Reprod Biol Endocrinol
JT  - Reproductive biology and endocrinology : RB&E
JID - 101153627
RN  - 3A64E3G5ZO (Bromocriptine)
RN  - 409J2J96VR (Androstenedione)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
RN  - 9002-62-4 (Prolactin)
RN  - EC 1.1.- (17-Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.- (20-Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.51 (3 (or 17)-beta-hydroxysteroid dehydrogenase)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - 17-Hydroxysteroid Dehydrogenases/blood/*metabolism
MH  - 20-Hydroxysteroid Dehydrogenases/blood/*metabolism
MH  - Androstenedione/blood
MH  - Animals
MH  - Bromocriptine/pharmacology
MH  - Corpus Luteum/*enzymology
MH  - Deciduoma/*physiology
MH  - Dopamine/metabolism
MH  - Estradiol/blood
MH  - Female
MH  - Luteal Phase/blood/physiology
MH  - Progesterone/biosynthesis/blood
MH  - Prolactin/biosynthesis/blood
MH  - Pseudopregnancy/blood/*enzymology
MH  - Rats
MH  - Rats, Wistar
PMC - PMC419377
EDAT- 2004/05/14 05:00
MHDA- 2005/07/27 09:00
PMCR- 2004/05/12
CRDT- 2004/05/14 05:00
PHST- 2004/03/11 00:00 [received]
PHST- 2004/05/12 00:00 [accepted]
PHST- 2004/05/14 05:00 [pubmed]
PHST- 2005/07/27 09:00 [medline]
PHST- 2004/05/14 05:00 [entrez]
PHST- 2004/05/12 00:00 [pmc-release]
AID - 1477-7827-2-22 [pii]
AID - 10.1186/1477-7827-2-22 [doi]
PST - epublish
SO  - Reprod Biol Endocrinol. 2004 May 12;2:22. doi: 10.1186/1477-7827-2-22.