PMID- 15140376
OWN - NLM
STAT- MEDLINE
DCOM- 20041124
LR  - 20061115
IS  - 1090-6576 (Print)
IS  - 1090-6576 (Linking)
VI  - 8
IP  - 1
DP  - 2004 Spring
TI  - Comparison of the validity of preimplantation genetic diagnosis for embryo 
      chromosomal anomalies by fluorescence in situ hybridization on one or two 
      blastomeres.
PG  - 69-72
AB  - Is it necessary to analyze two blastomeres in preimplantation genetic diagnosis 
      (PGD) by fluorescence in situ hybridization (FISH) or is one blastomere enough, 
      as suggested by some teams? We analyzed the sensitivity (Se), specificity (Sp), 
      positive predictive value (PPV), negative predictive value (NPV), false positives 
      (FP), false negatives (FN), and the efficiency (Eff) of FISH performed on one 
      (Group I) or two (Group II) blastomeres. Ninety embryos were analyzed (day 3), 19 
      blastocysts were replaced (day 5), 64 embryos were reanalyzed (day 5), (Group I = 
      23; Group II = 41). No differences were observed between the two groups for all 
      of the parameters considered, but one false negative was observed in Group I. 
      Furthermore, two embryos from Group II, which had a discordant diagnosis at PGD 
      (one blastomere being normal and one abnormal), were read as abnormal after 
      reanalysis. The accidental biopsy of the normal blastomere could have lead to the 
      selection of these 2 embryos for transfer, causing a misdiagnosis rate of 4.8%. 
      We conclude that embryo reanalysis is a useful tool to test the reliability of 
      PGD in each laboratory: that PGD on two blastomeres is safer because the practice 
      of PGD on one blastomere can result in a false-negative misdiagnosis.
FAU - Emiliani, Serena
AU  - Emiliani S
AD  - Fertility Clinic and Laboratory of Research on Human Reproduction, Free 
      University of Brussels, French Speaking, 1070 Brussels, Belgium. 
      semilian@ulb.ac.be
FAU - Gonzalez-Merino, Eric
AU  - Gonzalez-Merino E
FAU - Englert, Yvon
AU  - Englert Y
FAU - Abramowicz, Marc
AU  - Abramowicz M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Genet Test
JT  - Genetic testing
JID - 9802546
SB  - IM
MH  - *Blastomeres
MH  - *Chromosome Aberrations
MH  - False Negative Reactions
MH  - False Positive Reactions
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Pregnancy
MH  - *Preimplantation Diagnosis
EDAT- 2004/05/14 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/05/14 05:00
PHST- 2004/05/14 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/05/14 05:00 [entrez]
AID - 10.1089/109065704323016058 [doi]
PST - ppublish
SO  - Genet Test. 2004 Spring;8(1):69-72. doi: 10.1089/109065704323016058.