PMID- 15140869
OWN - NLM
STAT- MEDLINE
DCOM- 20040914
LR  - 20091119
IS  - 1355-4786 (Print)
IS  - 1355-4786 (Linking)
VI  - 10
IP  - 3
DP  - 2004 May-Jun
TI  - A cytogeneticist's perspective on genomic microarrays.
PG  - 221-6
AB  - The identification of cytogenetic imbalance is an important component of clinical 
      genetics. About 1 in 154 newborns has a chromosome abnormality. Conventional 
      cytogenetic analysis has enabled the identification of microscopic alterations of 
      the chromosomes. The development of fluorescence in situ hybridization (FISH) and 
      other molecular methodologies has made possible the identification of 
      submicroscopic aberrations. An additional development was comparative genomic 
      hybridization (CGH), a method that directly compares two genomes for DNA copy 
      differences. As first developed, the substrate for CGH analysis is normal 
      metaphase chromosomes. Recently, CGH has been applied to microarrays (array CGH) 
      constructed from large insert clones to identify chromosome imbalance. Array CGH 
      has many advantages over conventional cytogenetic and molecular cytogenetic 
      techniques. Array CGH can be comprehensive (genome-wide), high resolution, 
      amenable to automation, rapid, and sensitive. We anticipate that array CGH will 
      be employed in the clinical cytogenetics laboratory in the near future and will 
      lead to the identification of the chromosomal basis of new syndromes and existing 
      genetic conditions.
FAU - Shaffer, Lisa G
AU  - Shaffer LG
AD  - Health Research and Education Center, Washington State University Spokane, Sacred 
      Heart Medical Center, and Signature Genomic Laboratories, Spokane, Washington, 
      USA. lshaffer@wsu.edu
FAU - Bejjani, Bassem A
AU  - Bejjani BA
LA  - eng
PT  - Journal Article
PT  - Review
PL  - England
TA  - Hum Reprod Update
JT  - Human reproduction update
JID - 9507614
SB  - IM
MH  - *Chromosome Aberrations
MH  - Cytogenetics/*methods
MH  - Genetic Testing/methods
MH  - Genomics/*methods
MH  - Humans
MH  - *Oligonucleotide Array Sequence Analysis
RF  - 54
EDAT- 2004/05/14 05:00
MHDA- 2004/09/15 05:00
CRDT- 2004/05/14 05:00
PHST- 2004/05/14 05:00 [pubmed]
PHST- 2004/09/15 05:00 [medline]
PHST- 2004/05/14 05:00 [entrez]
AID - 10/3/221 [pii]
AID - 10.1093/humupd/dmh022 [doi]
PST - ppublish
SO  - Hum Reprod Update. 2004 May-Jun;10(3):221-6. doi: 10.1093/humupd/dmh022.