PMID- 15143175
OWN - NLM
STAT- MEDLINE
DCOM- 20040621
LR  - 20191120
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 24
IP  - 11
DP  - 2004 Jun
TI  - The homeodomain protein CDP regulates mammary-specific gene transcription and 
      tumorigenesis.
PG  - 4810-23
AB  - The CCAAT-displacement protein (CDP) has been implicated in developmental and 
      cell-type-specific regulation of many cellular and viral genes. We previously 
      have shown that CDP represses mouse mammary tumor virus (MMTV) transcription in 
      tissue culture cells. Since CDP-binding activity for the MMTV long terminal 
      repeat declines during mammary development, we tested whether binding mutations 
      could alter viral expression. Infection of mice with MMTV proviruses containing 
      CDP binding site mutations elevated viral RNA levels in virgin mammary glands and 
      shortened mammary tumor latency. To determine if CDP has direct effects on MMTV 
      transcription rather than viral spread, virgin mammary glands of homozygous 
      CDP-mutant mice lacking one of three Cut repeat DNA-binding domains (DeltaCR1) 
      were examined by reverse transcription-PCR. RNA levels of endogenous MMTV as well 
      as alpha-lactalbumin and whey acidic protein (WAP) were elevated. Heterozygous 
      mice with a different CDP mutation that eliminated the entire C terminus and the 
      homeodomain (DeltaC mice) showed increased levels of MMTV, beta-casein, WAP, and 
      alpha-lactalbumin RNA in virgin mammary glands compared to those from wild-type 
      animals. No differences in amounts of WDNM1, epsilon-casein, or 
      glyceraldehyde-3-phosphate dehydrogenase RNA were observed between the 
      undifferentiated mammary tissues from wild-type and mutant mice, indicating the 
      specificity of this effect. These data show independent contributions of 
      different CDP domains to negative regulation of differentiation-specific genes in 
      the mammary gland.
FAU - Zhu, Quan
AU  - Zhu Q
AD  - Section of Molecular Genetics and Microbiology, The University of Texas at 
      Austin, One University Station, A5000, 24th and Speedway, ESB 226, Austin, TX 
      78712-0162, USA.
FAU - Maitra, Urmila
AU  - Maitra U
FAU - Johnston, Dennis
AU  - Johnston D
FAU - Lozano, Mary
AU  - Lozano M
FAU - Dudley, Jaquelin P
AU  - Dudley JP
LA  - eng
GR  - CA34780/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (Cux1 protein, mouse)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Repressor Proteins)
SB  - IM
MH  - Animals
MH  - Binding Sites
MH  - Gene Expression Regulation, Neoplastic/*physiology
MH  - Gene Transfer Techniques
MH  - Genetic Vectors
MH  - Homeodomain Proteins
MH  - Mammary Glands, Animal/*metabolism
MH  - Mammary Neoplasms, Animal/*metabolism
MH  - Mammary Tumor Virus, Mouse
MH  - Mice
MH  - Nuclear Proteins/genetics/*metabolism
MH  - Rats
MH  - Repressor Proteins/genetics/*metabolism
PMC - PMC416401
EDAT- 2004/05/15 05:00
MHDA- 2004/06/24 05:00
PMCR- 2004/06/01
CRDT- 2004/05/15 05:00
PHST- 2004/05/15 05:00 [pubmed]
PHST- 2004/06/24 05:00 [medline]
PHST- 2004/05/15 05:00 [entrez]
PHST- 2004/06/01 00:00 [pmc-release]
AID - 24/11/4810 [pii]
AID - 1304-03 [pii]
AID - 10.1128/MCB.24.11.4810-4823.2004 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2004 Jun;24(11):4810-23. doi: 10.1128/MCB.24.11.4810-4823.2004.