PMID- 15144400
OWN - NLM
STAT- MEDLINE
DCOM- 20041207
LR  - 20061115
IS  - 1320-5463 (Print)
IS  - 1320-5463 (Linking)
VI  - 54
IP  - 6
DP  - 2004 Jun
TI  - Abnormalities in chromosome 17 and p53 in lung carcinoma cells detected by 
      fluorescence in situ hybridization.
PG  - 413-9
AB  - The value of fluorescence in situ hybridization (FISH) as an aid to deciding the 
      prognosis for lung carcinoma patients, comparing quantitatively the signal from 
      the p53 gene (17p13.1) on chromosome 17, was studied. A dual-labeling technique 
      was used, using probes for the centromeric region of chromosome 17 and for the 
      p53 gene locus. FISH was used on frozen sections of 68 surgically resected lung 
      carcinoma (20 adenocarcinoma; 37 squamous cell carcinoma; 11 large, small, and 
      other cell carcinoma). Hybridization signals were counted for 100-200 interphase 
      nuclei per specimen using a Zeiss confocal laser scanning microscope (Carl Zeiss, 
      Oberkochen, Germany) equipped with a bandpass filter for diaminophenolindole and 
      a longpass filter for rhodamine. Clinicopathologic data were evaluated using the 
      Statistical Analysis System. Chromosome 17 polysomy (three or more signals) was 
      greater in poorly differentiated than in well-differentiated lung carcinoma (P < 
      0.05). p53 deletion correlated with p53 immunostaining (P < 0.05). Thus, analysis 
      by FISH using DNA probes for chromosome 17 and p53 may be of some, albeit 
      limited, value in determination of prognosis.
FAU - Kawai, Toshiaki
AU  - Kawai T
AD  - Department of Pathology, National Defense Medical College, Tokorozawa, Japan. 
      tkawai@cc.ndmc.ac.jp
FAU - Hiroi, Sadayuki
AU  - Hiroi S
FAU - Nakanishi, Kuniaki
AU  - Nakanishi K
FAU - Sakurai, Yutaka
AU  - Sakurai Y
FAU - Torikata, Chikao
AU  - Torikata C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Australia
TA  - Pathol Int
JT  - Pathology international
JID - 9431380
SB  - IM
MH  - Carcinoma/*genetics/pathology/surgery
MH  - Cell Nucleus/genetics/pathology
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 17
MH  - Female
MH  - *Genes, p53
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Interphase
MH  - Lung Neoplasms/*genetics/pathology/surgery
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Prognosis
EDAT- 2004/05/18 05:00
MHDA- 2004/12/16 09:00
CRDT- 2004/05/18 05:00
PHST- 2004/05/18 05:00 [pubmed]
PHST- 2004/12/16 09:00 [medline]
PHST- 2004/05/18 05:00 [entrez]
AID - PIN1635 [pii]
AID - 10.1111/j.1440-1827.2004.01635.x [doi]
PST - ppublish
SO  - Pathol Int. 2004 Jun;54(6):413-9. doi: 10.1111/j.1440-1827.2004.01635.x.