PMID- 1514589
OWN - NLM
STAT- MEDLINE
DCOM- 19920925
LR  - 20171213
IS  - 0002-9513 (Print)
IS  - 0002-9513 (Linking)
VI  - 263
IP  - 2 Pt 1
DP  - 1992 Aug
TI  - TGF-beta 1 potentiates growth factor-stimulated proliferation of vascular smooth 
      muscle cells in genetic hypertension.
PG  - C420-8
AB  - We have examined the interactions between transforming growth factor-beta 1 
      (TGF-beta 1) and epidermal growth factor (EGF), basic fibroblast growth factor 
      (bFGF), or platelet-derived growth factor (PDGF) isoforms PDGF-AB and PDGF-BB on 
      the proliferation of vascular smooth muscle cells isolated from the spontaneously 
      hypertensive rat. TGF-beta 1 alone stimulated [3H]thymidine incorporation 
      approximately twofold without a corresponding increase in cell number. In 
      combination, TGF-beta 1 action was synergistic in further stimulating both DNA 
      synthesis and cell proliferation 100-300% above the responses elicited by each 
      growth factor. To gain further insight into the mechanism responsible for this 
      potentiation, we examined the interaction between TGF-beta 1 and EGF. The 
      synergistic interaction between TGF-beta 1 and EGF on DNA synthesis was 
      independent of initial cell density. This effect of TGF-beta 1 was initiated 
      early in the G1 phase of the cell cycle and did not appear to be mediated through 
      the mobilization of Ca2+ or alterations in c-jun mRNA expression. However, in the 
      presence of both TGF-beta 1 and EGF, there was a sustained elevation of c-myc 
      mRNA levels over a 24-h period. These results suggest that TGF-beta 1 may 
      interact with other growth factors in vivo to enhance their proliferative action 
      on vascular smooth muscle of spontaneously hypertensive rats via mechanisms 
      dependent on c-myc mRNA expression.
FAU - Saltis, J
AU  - Saltis J
AD  - Baker Medical Research Institute, Alfred Hospital, Prahran, Victoria, Australia.
FAU - Agrotis, A
AU  - Agrotis A
FAU - Bobik, A
AU  - Bobik A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Physiol
JT  - The American journal of physiology
JID - 0370511
RN  - 0 (Growth Substances)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Animals
MH  - Cell Communication
MH  - Cell Cycle/drug effects
MH  - Cell Division/drug effects
MH  - DNA/biosynthesis
MH  - Growth Substances/*pharmacology
MH  - Hypertension/genetics/metabolism/*pathology
MH  - Male
MH  - Muscle, Smooth, Vascular/drug effects/metabolism/*pathology
MH  - Proto-Oncogene Proteins c-myc/genetics
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Signal Transduction
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 1992/08/01 00:00
MHDA- 1992/08/01 00:01
CRDT- 1992/08/01 00:00
PHST- 1992/08/01 00:00 [pubmed]
PHST- 1992/08/01 00:01 [medline]
PHST- 1992/08/01 00:00 [entrez]
AID - 10.1152/ajpcell.1992.263.2.C420 [doi]
PST - ppublish
SO  - Am J Physiol. 1992 Aug;263(2 Pt 1):C420-8. doi: 10.1152/ajpcell.1992.263.2.C420.