PMID- 15147040
OWN - NLM
STAT- MEDLINE
DCOM- 20040702
LR  - 20191108
IS  - 0914-7470 (Print)
IS  - 0914-7470 (Linking)
VI  - 16
IP  - 4
DP  - 2003 Dec
TI  - Ex vivo enhancement of antigen-presenting function of dendritic cells and its 
      application for DC-based immunotherapy.
PG  - 199-204
AB  - Dendritic cells (DCs) are potent antigen presenting cells that are able to 
      initiate and modulate immune responses and are hence exploited as cellular 
      vaccines for immunotherapy. In particular DCs generated from peripheral blood 
      monocytes (Mo-DCs) have been used with promising results as a new approach for 
      the immunotherapy of cancer. In this study, we have analyzed the changes in the 
      pattern of expression molecules on Mo-DCs during DC maturation using different 
      maturation cytokine combinations and the expansion capacity of an antigen 
      specific CD8+T cells monitored by flow cytometry with the fluorescent tetramers 
      and anti-CD8 monoclonal antibody. These analyses revealed that the expansion of 
      antigen specific CD8+T cells is the most effective when T cells were activated by 
      fully maturated DCs by culturing monocytes for 5 days in the presence of GM-CSF 
      and IL-4, followed by 2-3 days of maturation with pro-inflammatory mediators 
      including TNFalpha, IL-6, IL-1beta and PGE2. These results pave the way to a more 
      effective immunotherapy using DCs for patients with malignancy, as well as 
      infectious diseases.
FAU - Nieda, Mie
AU  - Nieda M
AD  - Medinet, Institute of Molecular Immunology, Tokyo, Japan. nieda@medinet-inc.co.jp
FAU - Tomiyama, Mai
AU  - Tomiyama M
FAU - Egawa, Kohji
AU  - Egawa K
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Hum Cell
JT  - Human cell
JID - 8912329
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (Cytokines)
RN  - 0 (Peptides)
SB  - IM
MH  - *Antigen Presentation
MH  - Antigens, Neoplasm/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Cytokines/therapeutic use
MH  - Cytotoxicity, Immunologic
MH  - Dendritic Cells/*immunology/transplantation
MH  - Humans
MH  - Immunotherapy, Adoptive/*methods
MH  - Lymphocyte Activation
MH  - Monocytes/cytology
MH  - Neoplasms/immunology/therapy
MH  - Peptides/therapeutic use
EDAT- 2004/05/19 05:00
MHDA- 2004/07/03 05:00
CRDT- 2004/05/19 05:00
PHST- 2004/05/19 05:00 [pubmed]
PHST- 2004/07/03 05:00 [medline]
PHST- 2004/05/19 05:00 [entrez]
AID - 10.1111/j.1749-0774.2003.tb00154.x [doi]
PST - ppublish
SO  - Hum Cell. 2003 Dec;16(4):199-204. doi: 10.1111/j.1749-0774.2003.tb00154.x.