PMID- 15147347
OWN - NLM
STAT- MEDLINE
DCOM- 20040625
LR  - 20181113
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 136
IP  - 3
DP  - 2004 Jun
TI  - Constitutive expression of interleukin-18 and interleukin-18 receptor mRNA in 
      tumour derived human B-cell lines.
PG  - 456-62
AB  - Interleukin-18 (IL-18) is a pro-inflammatory cytokine involved in the Th1 immune 
      response and expressed by a variety of cell types. IL-18 is a member of the IL-1 
      family and plays an important role in autoimmune diseases and inflammation. Using 
      reverse transcriptase-polymerase chain reaction (RT-PCR) mRNA expression of 
      IL-18, IL-18 receptor alpha (IL-18R alpha), and beta (IL-18R beta) were studied 
      in tumour derived human B-cell lines. Furthermore, we investigated IL-18 protein 
      secretion by using enzyme linked immunosorbent assay (ELISA). The results, as 
      presented in this report, suggest that IL-18, IL-18R alpha, and IL-18R beta mRNA 
      are constitutively and ubiquitously expressed in human B-cell lines, but 
      secretion of the functional protein does not occur. We therefore speculate that 
      IL-18 possibly affects B-cells through paracrine actions.
FAU - Lorey, S L
AU  - Lorey SL
AD  - Laboratory of Cytokine Research, Department of Biology, University of West 
      Florida, Pensacola, Florida 32514, USA.
FAU - Huang, Y C
AU  - Huang YC
FAU - Sharma, V
AU  - Sharma V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (IL18R1 protein, human)
RN  - 0 (Interleukin-18)
RN  - 0 (Interleukin-18 Receptor alpha Subunit)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Interleukin)
RN  - 0 (Receptors, Interleukin-18)
SB  - IM
MH  - B-Lymphocytes/*immunology
MH  - Cell Line, Tumor
MH  - Humans
MH  - Interleukin-18/*genetics
MH  - Interleukin-18 Receptor alpha Subunit
MH  - RNA, Messenger/*analysis
MH  - Receptors, Interleukin/*genetics
MH  - Receptors, Interleukin-18
MH  - Reverse Transcriptase Polymerase Chain Reaction
PMC - PMC1809060
EDAT- 2004/05/19 05:00
MHDA- 2004/06/26 05:00
PMCR- 2005/06/01
CRDT- 2004/05/19 05:00
PHST- 2004/05/19 05:00 [pubmed]
PHST- 2004/06/26 05:00 [medline]
PHST- 2004/05/19 05:00 [entrez]
PHST- 2005/06/01 00:00 [pmc-release]
AID - CEI2465 [pii]
AID - 10.1111/j.1365-2249.2004.02465.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2004 Jun;136(3):456-62. doi: 10.1111/j.1365-2249.2004.02465.x.